Mus musculus
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA311700 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR3161910.fastq.gz | Fastqsanger.gz | |||
| SRR3161911.fastq.gz | Fastqsanger.gz | |||
| SRR3161906.fastq.gz | Fastqsanger.gz | |||
| SRR3161907.fastq.gz | Fastqsanger.gz | |||
| SRR3161908.fastq.gz | Fastqsanger.gz |
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Project description:Mutations in the enzymes IDH1 and IDH2 have been identified in a wide variety of tumors like glioma, chondrosarcoma, thyroid cancer, lymphoma, melanoma, and in acute myeloid leukemia. Mutated IDH1/2 produces the metabolite 2-hydroxyglutarate (2HG), which interferes with epigenetic regulation of gene expression, and thus may promote tumorigenesis. HoxA9 immortalised bone marrow cells from C57BJ/6 mice were tranduced with either empty vector or pSF91-IDH1wt-IRES-EGFP, or pSF91-IDH1mut-IRES-GFP and transplanted in irradiated recipient mice. Alternatively, HoxA9 immortalised cells transduced with empty vector transplanted mice were treated with R-2HG at dose of 1mg/day for four weeks. Four weeks after transplantation/treatment GFP+ cells were sorted from mouse bone marrow, from which total RNA ws extracted and subjected to microarray analysis.
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Project description:Gene expression changes induced by overexpression of IDH1mut in the sorted mouse bone marrow cells
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