Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Chlorophyte and Charophyte green algae

ABSTRACT: Raw Illumina reads and transcriptome assemblies of Chlorella sorokiniana, Closteriopsis acicularis, Cosmarium turpinii, Pandorina charkowiensis, Scenedesmus acuminatus, Selenastrum capricornotum, Staurastrum punctulatum and Tetraedron minimum. In add

PROVIDER: PRJNA312560 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR3478580_1.fastq.gz	Fastqsanger.gz
	SRR3478580_2.fastq.gz	Fastqsanger.gz
	SRR3478590_1.fastq.gz	Fastqsanger.gz
	SRR3478590_2.fastq.gz	Fastqsanger.gz
	SRR3478620_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 216

Similar Datasets

miRNA transcriptome profiling of Chlorella sorokiniana and Chlorella zofingiensis under normal and stress condition

Project description:Purpose: The purpose of this study are to identify the miRNA involved in regulating the production of metabolites in Chlorella sorokiniana and Chlorella zofingiensis under normal and stress-induced condition through RNA-sequencing technique. Methods: miRNA transcriptome profile from normal and stress sample of C. sorokiniana and C. zofingiensis were generated, in triplicate, using Illumina Miseq. The sequence reads that passed quality filters were analysed using CLC genomic workbench and OmiRas. Results: The known and predicted novel miRNAs were identified. Although most of the identified miRNAs were not functionally determined, this study suggests that they were species-specific, which may have roles in regulating genes during stress related condition.

2022-10-01 | GSE107372 | GEO

Genome sequences of Chlorella sorokiniana UTEX 1602 and Micractinium conductrix SAG 241.80: implications to maltose excretion by a green alga

Project description:Micractinium conductrix SAG 241.80 secretes sugars (especially maltose) at pH 5.7 while very little sugar is found at pH 7.6. Another algae, Chlorella sorokiniana does not secrete sugars at either pH. We evaluated the transcriptional profiles of both species of algae at pH 5.7 and 7.6 to make comparisons in the regulation of potential genes involved in these pathways.

2017-12-27 | GSE98781 | GEO

Nitrogen starvation leads to TOR kinase-mediated downregulation of fatty acid synthesis in the algae Chlorella sorokiniana and Chlamydomonas reinhardtii

Project description:The accumulation of triacylglycerol (TAG) as a storage compound in eukaryotic algae has been the subject of extensive studies over the last 25 years. The model industrial alga Chlorella sorokiniana accumulates TAG and other storage compounds under nitrogen (N)-limited growth. Previously we used transcriptomics to explore the regulation of TAG synthesis in C. sorokiniana. Surprisingly, our analysis showed that the expression of several key genes encoding enzymes involved in plastidic fatty acid synthesis are significantly repressed. Metabolic labeling with radiolabeled acetate showed that de novo fatty acid synthesis is indeed downregulated under N-limitation. Likewise, inhibition of the Target of Rapamycin kinase (TOR), a key regulator of metabolism and growth, decreased fatty acid synthesis. We compared the changes induced in the quantitative proteome and phosphoproteome of cells under N-limitation or TOR inhibition and found extensive overlap between the N-limited and TOR-inhibited conditions. We also detected variations in the phosphorylation of TOR complex proteins under N-limitation, indicating that TOR signaling is altered. Our results indicate that under N-limitation there is significant metabolic remodeling, including fatty acid synthesis, mediated by TOR signaling. We find that TOR-mediated metabolic remodeling of fatty acid synthesis under N-limitation is conserved in the chlorophyte algae Chlorella sorokiniana and Chlamydomonas reinhardtii.

2024-10-17 | PXD041691 | Pride

Evaluation of the potential of 4 micro algae extracts to modulate the immune response and metabolic state in intestinal epithelium cells

Project description:Micro algae's are used as alternative protein source in human and animal diets. Besides micro algae contain substantial amounts of proteins they also contain a high concentration of, often unique, biological and chemical substances with potential to induce beneficial and health promoting effects in humans and animals. This study was set up to evaluate the potential of these substances to improve (intestinal) health. The effect of extracts prepared from 3 monocultures of micro algae's (Chlorella vulgaris [C], Haematococcus pluvialis [H], and Spirulina platensis [S]) and a mixed culture of micro algae's (AM; a mixture of Scenedesmus sp. and Chlorella sp. ) was studied in the presence and absence of the enterotoxigenic bacterium Escherichia coli k99 strain (ETEC, [E]) as an in vitro challenge. The E.coli-k99 strain with adhesion factor F41 (41/32) was isolated from a mastitis-infected udder. Gene expression was measured in cultured intestinal porcine epithelium cells (IPECJ2 cell line) after 2 and 6 hours incubation with C, H, and S extracts, and after 6 hours with the AM extract, using “whole genome” porcine microarrays. Gene expression profiles were analysed using functional bioinformatics programs to provide insight in the biological processes induced by micro algae extracts.

2019-03-14 | GSE113365 | GEO

Effect of cadmium in the microalga Chlorella sorokiniana: A proteomic study

Project description:Effect of cadmium in the microalga Chlorella sorokiniana

2020-10-05 | PXD015932 | Pride

Transcriptome profiling of Chlorella sorokiniana under normal and stress condition

Project description:Purpose: The purpose of this study are to identify the genes involved in regulating the production of metabolites in Chlorella sorokiniana under stress-induced condition through RNA-sequencing technique. Methods:Transcriptome profile from normal and stress sample of C. sorokiniana were generated, in triplicate, using Illumina Hiseq2000. The sequence reads that passed quality filters were de-novo assembled using Trinity doftware, followed by analysis using Burrows–Wheeler Aligner (BWA) and DESeq2. Lastly, RT-qPCR was used to validate the expression level of genes. Results: A total of 200 million reads was obtained from both normal and stress induced samples of C. sorokiniana. The assembly program produced 18,310 assembled transcripts with a length ranging from 165 to 16,695 bp. Differential expression analysis revealed 933 transcripts were significantly differentially expressed between normal and stress with 431 were up-regulated and 502 were down-regulated. Pathway analysis revealed that the differentially expressed genes were associated with a variety of KEGG pathways.

2020-10-20 | GSE105427 | GEO

Chlorella sorokiniana

Project description:Chlorella sorokiniana

| PRJNA791694 | ENA

Tetradesmus lagerheimii

Project description:Scenedesmus acuminatus Raw sequence reads

| PRJNA851546 | ENA

Increased microalgae Chlorella sorokiniana lipid yield induced by low-dose ionizing radiation

Project description:We demonstrate that low-dose ionizing radiation from X-rays drives metabolic activation in microalgae. We exploited this phenomenon to develop a method for increased lipid yield in stationary phase Chlorella sorokiniana cultures by 25% in just 24 hours, caused by a reproducible metabolic response that includes up-regulation of >30 lipid metabolism genes. This approach avoids the need to modify the strain or cultivation conditions, and does not affect cell viability or biomass.

2024-06-06 | E-MTAB-12288 | biostudies-arrayexpress