Project description:The purpose is to obtain samples for mRNA analysis in human liver Huh cells using the following viruses obtained by reverse genetics: Zaire Ebola virus wild-type (WT) in the ΔVP30 background (wild).
Project description:The purpose is to obtain samples for mRNA analysis in human liver Huh cells using the following viruses obtained by reverse genetics: Zaire Ebola virus wild-type (WT) in the ΔVP30 background (wild), ΔsGP virus (eliminates expression of soluble glycoprotein [GP]) (ssGP), and Δmucin virus (encodes a GP lacking the mucin domain) (mucin).
Project description:The purpose is to obtain samples for microRNA analysis in human liver Huh cells using the following viruses obtained by reverse genetics: Zaire Ebola virus wild-type (WT) in the ΔVP30 background (wild), ΔsGP virus (eliminates expression of soluble glycoprotein [GP]) (ssGP), and Δmucin virus (encodes a GP lacking the mucin domain) (mucin).
Project description:The purpose is to obtain samples for mRNA analysis in human U937 cells infected with Zaire Ebola virus wild-type in the ΔVP30 background and Δmucin virus.
Project description:The purpose is to obtain samples for microRNA analysis in human U937 cells infected with Zaire Ebola virus wild-type in the ΔVP30 background and Δmucin virus.
Project description:The purpose is to obtain samples for mRNA analysis in HUV-EC-C cells, a human endothelial cell line isolated from the vein of the umbilical cord, using the following viruses obtained by reverse genetics: Zaire Ebola virus wild-type in the ΔVP30 background (deltaVP30-WT) and mutant lacking the mucin domain (deltaVP30-deltamucin).
Project description:The purpose is to obtain HUH VP30 samples for mRNA analysis with and with out interferon α and β treatment. Huh VP30 cells have been stably transfected with the Ebola VP30 gene, enabling replication of VP30-deficient Ebola virus. PubMed ID 18212124 and 19211761.
Project description:The purpose of this experiment was to obtain samples for mRNA analysis in IHH cells infected with Zaire Ebola virus and mutants: Zaire Ebola virus: This wild-type Ebola virus - strain Mayinga - was isolated from a fatal human case in Zaire (now known as the Democratic Republic of Congo) in 1976 Zaire Ebola virus, VP35 R312A possesses a R312A mutation in the VP35 protein. Zaire Ebola virus, delta sGP. Lacks the ability to produce non-structural protein, the secreted glycoprotein (sGP). Zaire Ebola virus, delta mucin. Lacks the mucin-like domain (MLD), which contains both N-linked and O-linked glycosylation sites, for the glycoproteins.
