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Porphyromonas gingivalis

ABSTRACT: Adaptation of Porphyromonas gingivalis to Streptococcus gordonii observed using time-coursed RNA-Seq

PROVIDER: PRJNA318514 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR3384370.fastq.gz	Fastqsanger.gz
	SRR3384380.fastq.gz	Fastqsanger.gz
	SRR3384390.fastq.gz	Fastqsanger.gz
	SRR3384371.fastq.gz	Fastqsanger.gz
	SRR3384381.fastq.gz	Fastqsanger.gz

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Adaptation of Porphyromonas gingivalis to Streptococcus gordonii observed using time-coursed RNA-Seq

Project description:Periodontal diseases are one of the most common human maladies and appear to be caused by the interaction of proximal pathogens such as Porphyromonas gingivalis but only as part of the polymicrobial community known as dental plaque. Streptococcus gordonii is an early colonizing oral organism that binds to oral surfaces and provides adherence for organisms such as P. gingivalis. Together P. gingivalis and S. gordonii form one of the simplest models of potentially pathogenic dental plaque. We used RNA sequencing to monitor the transcriptome of P. gingivalis over time in a biofilm model both in the presence and absence of S. gordonii. Samples were taken at 5, 30, 120, 240, and 360 minutes after shifing from planktonic to sessile conditions and growth media to PBS. When compared to planktonic cells increased transcripts were found for stress, amino acid catabolism, and comeptence and decreased transcripts for DNA replication. The presence of S. gordonii resulted in fewer changes from planktonic cells implying physiological support to Pl gingivalis making the transition from planktonic to sessile easier.

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Project description:Role of RNA-binding protein, Pgr, in Porphyromonas gingivalis.

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Pathogenesis research of tumorigenesis and progression of colorectal cancer modulated by gingipains secreted by Porphyromonas gingivalis

Project description:Primary outcome(s): Abundance of Porphyromonas gingivalis (Pg) in samples Study Design: Case-Control study

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Comparison of Porphyromonas gingivalis W83 gene expression when grown with or without galactose

Project description:Porphyromonas gingivalis (P. gingivalis) W83 cultures in early exponential phase were supplemented with 0.1% galactose or vehicle control. The cultures were then permitted to grow anaerobically at 37°C for 3 hours, which corresponds to approximately one doubling of P. gingivalis. At this point, RNA extraction was performed using the cultures, and these samples were processed and submitted for RNA sequencing using an Illumina platform. Modest changes in gene expression were observed between cultures grown with or without galactose, and the majority of changes were associated with processes occurring at the cell membrane.

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