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Homo sapiens

ABSTRACT: Genome-wide H3K4me3 after MK2206/DMSO treatment of T47D cells

PROVIDER: PRJNA319401 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR3421130.fastq.gz	Fastqsanger.gz
	SRR3421131.fastq.gz	Fastqsanger.gz
	SRR3421122.fastq.gz	Fastqsanger.gz
	SRR3421132.fastq.gz	Fastqsanger.gz
	SRR3421123.fastq.gz	Fastqsanger.gz

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Homo sapiens

Project description:Genome-wide KDM5A occupancy after MK2206/DMSO treatment of T47D cells

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Genome-wide H3K4me3 after MK2206/DMSO treatment of T47D cells

Project description:We perfomed ChIP-seq using H3K4me3 antibodies in T47D cells after 24 hour treatment with the AKT inhibitor MK2206 or DMSO. We demonstrate that at a selected group of loci H3K4me3 is affected by AKT inhibition. Comparison of genome-wide H3K4me3 binding after AKT inhibition vs vehicle

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Genome-wide H3K4me3 after MK2206/DMSO treatment of T47D cells

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Project description:Messenger RNA expression after MK2206/DMSO treatment of T47D cells

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Genome-wide KDM5A occupancy after MK2206/DMSO treatment of T47D cells

Project description:We perfomed ChIP-seq using KDM5A antibodies in T47D cells after 24 hour treatment with the AKT inhibitor MK2206 or DMSO. We show that the cistrome of the H3K4 demethylase KDM5A is affected by AKT inhibition. Comparison of genome-wide KDM5A binding after AKT inhibition vs vehicle

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Messenger RNA expression after MK2206/DMSO treatment of T47D cells

Project description:We performed RNA-seq in T47D cells after 24 hour treatment with the AKT inhibitor MK2206 or DMSO. We show that a group of transcripts is differentailly expressed after AKT inhibition.

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Genome-wide KDM5A occupancy after MK2206/DMSO treatment of T47D cells

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Project description:We report high-throughput profiling of RBM39 and MLL1 chromatin occupancy; H3K4me3 marks in T47D Control, T47D RBM39 KD and PME cells. By obtaining over four billion bases of sequence from chromatin immunoprecipitated DNA, we generated genome-wide chromatin-state maps of RBM39, MLL1 and H3K4me3 in T47D and PME cells. We found that more than 4500 regions were cofound by RBM39 and MLL1.

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