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Whole transcriptome analysis of functionally distinct cell types of the jejunal intestinal epithelium of germfree and conventionalized mice

ABSTRACT: Whole transcriptome analysis of functionally distinct cell types of the jejunal intestinal epithelium of germfree and conventionalized mice

PROVIDER: PRJNA320643 | ENA |

REPOSITORIES: ENA

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Whole transcriptome analysis of functionally distinct cell types of the jejunal intestinal epithelium of germfree and conventionalized mice [mRNA]

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Project description:Whole transcriptome analysis of functionally distinct cell types of the jejunal intestinal epithelium of germfree and conventionalized mice [mRNA]

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Whole transcriptome analysis of functionally distinct cell types of the jejunal intestinal epithelium of germfree and conventionalized mice

Project description:This SuperSeries is composed of the SubSeries listed below.

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Project description:Intestinal epithelium development is dramatically impaired in germfree rodents, but the consequences of the absence of microbiota have been overlooked in other epithelia. In the present study, we present the first description of the bacterial communities associated with the olfactory epithelium and explored differences in olfactory epithelium characteristics between germfree and conventional, specific pathogen-free, mice. While the anatomy of the olfactory epithelium was not significantly different, we observed a thinner olfactory cilia layer along with a decreased cellular turn-over in germfree mice. Using electro-olfactogram, we recorded the responses of olfactory sensitive neuronal populations to various odorant stimulations. We observed a global increase in the amplitude of responses to odorants in germfree mice as well as altered responses kinetics. These changes were associated with a decreased transcription of most olfactory transduction actors and of olfactory xenobiotic metabolising enzymes. Overall, we present here the first evidence that the microbiota modulates the physiology of olfactory epithelium. As olfaction is a major sensory modality for most animal species, the microbiota may have an important impact on animal physiology and behaviour through olfaction alteration.

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