Project description:We report the application of dual RNA-sequencing technology for high-throughput profiling of histone modifications in HaCat cells and Trichophyton mentagrophytes complex.For co-culture assays, a ratio of 2.5×105 cells/mL of keratinocytes to 2.5×105 conidia/mL of T. mentagrophytes, T. interdigitale, and T. tonsurans solution were used (MOI=1). The experiment was carried out for 24 h in a humidified incubator maintained at 37 ºC . We used dual RNA-seq to study the different host immune responses against the T. mentagrophytes complex and we the transcriptional profiles of differentially expressed genes in dermatophytes.
Project description:Our study provides considerable gene expression information of Trichophyton mentagrophytes at the transcriptional level, which will help accelerate the research on the antifungal medicine development. Additionally, we have demonstrated the feasibility of using the Illumina sequencing based DGE system for gene expression profiling, and have shed new light on functional studies of the genes involved in antifungal mechanisms of berberine hydrochloride and clotrimazole.
Project description:Under the action of Trichophyton 1000 UG / ml, the colony of Trichophyton mentagrophyte was completely inhibited. The spore number and germination rate of Trichophyton mentagrophyte under the action of 100ug / ml and 10ug / ml were significantly lower than those in the control group. Under the action of Trichophyton, the mitochondria in the mycelium of Trichophyton mentagrophyte were cleaved. Under the action of trichomycin, the related genes in mitochondria decreased significantly. This showed that mitochondria were obviously damaged during trichomycin treatment. It is speculated that Trichophyton can cause mitochondrial damage and reduce the efficiency of respiratory chain, but Trichophyton can synthesize enough ATP by regulating related ATP synthase to resist the invasive effect caused by Trichophyton stimulation.
