Project description:This dataset relates to a proteomic analysis of Ave1 homologs in the fungal pathogens Venturia pirina and Venturia inequalis. These species are the cause of scab disease on European pear (Pyrus communis) and apple (Malus pumila). Sample preparation for both V. pirina and V. inequalis was designed to focus on effectors and eliminate host proteins. To do this the fungi were grown in vitro, on cellophane sheets mimicing the growth habit in infected leaves and proteins were extracted using a gentle washing procedure designed to avoid cell wall breakage. LC-MS/MS data was queried against a protein database generated from gene predictions obtained from whole genome sequences of V. pirina and V. inequalis.
Project description:Induction of whole genome gene expression level changes in leaves of grafted apple genotypes exhibiting contrasted QTL architecture (from one to a combination of three QTL) after the inoculation of the pathogenic fungus Venturia inaequalis
Project description:This dataset relates to a proteogenomic analysis for Venturia pirina, a fungus that causes scab disease on European pear (Pyrus communis). V. pirina is host specific and infection is thought to be mediated by small, secreted effector proteins. To focus on these effectors we analysed extracts from V. pirina grown in vitro, on cellophane sheets, that mimics the growth habit in infected leaves. LC-MS/MS data was queried against a protein database generated by combining in-silico predicted transcripts with six-frame translations of a whole genome sequence for V. pirina.
