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Tobacco etch virus strain:TEV-eGFP

ABSTRACT: Experimental evolution of Tobacco etch virus carrying eGFP

PROVIDER: PRJNA321664 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR3528220_1.fastq.gz	Fastqsanger.gz
	SRR3528220_2.fastq.gz	Fastqsanger.gz
	SRR3528230_1.fastq.gz	Fastqsanger.gz
	SRR3528230_2.fastq.gz	Fastqsanger.gz
	SRR3528221_1.fastq.gz	Fastqsanger.gz

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Tobacco etch virus strain:TEV-eGFP

Project description:Multi-host experimental evolution of Tobacco etch virus carrying eGFP

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Project description:In this experiment, Arabidopsis plants infected by a virus, Tobacco etch virus (TEV), a potyvirus, were compared with healthy plants to identify genes for which the expression is modified by the viral infection. Analysis of both inoculated leaves and upper young leaves were performed 7 days after the inoculation with the virus (or with only buffer for the healthy plants).

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Project description:Transcription profiling of Arabidopsis plants infected with the Tobacco etch potyvirus TEV and the evolved TEV-At17, which was generated by serial passes of ancestral TEV in Arabidopsis. Infection with TEV-At17 cause severe symptoms in the plant, compared with the asymptomatic TEV-infection. Keywords: Virus infection Five replicates for each sample category (Arabidopsis Ler infected with mock, or with TEV and TEV-At17 viruses) were generated, and compared with a global reference, generated from an equimolar mix of amplified RNAs from each of the 15 plants. Three replicas (#1,2, and 3) in each group were labeled with Cy5 and compared with Cy3-labeled reference mix, while the other two replicas (#4 and 5) were reversed-labeled.

2008-06-23 | E-GEOD-11088 | biostudies-arrayexpress

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Project description:Experimental evolution of alternative gene orders in Tobacco etch virus

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The transcriptomics of an evolved plant-virus gene-for-gene interaction

Project description:We performed threefold evolution experiment with tobacco etch potyvirus isolate At17b (TEV-At17b) in five different ecotypes of Arabidopsis thaliana L. (Di-2, Ei-2, Ler-0, St-0, and Wt-1,), that are heterogeneous in their susceptibility to TEV-At17b infection. After the evolution phase, (i) we characterized the fitness and virulence of the evolved lineages across all host ecotypes, (ii) the molecular changes fixed in the viral genomes. Next, we analyzed the transcriptoma of the plants using Agilent Microarray tecnology (i)plants infected with local adapted viral lineage, (ii)plants infected with evolved virus vs. ancestal virus, (iii) virus evolved in new host and infecting its original host, (iv) transcriptoma of most specialized and most generalized viruses infecting all five ecotypes.

2016-04-18 | GSE66020 | GEO

Transcript profiling of different Arabidopsis thaliana ecotypes in response to Tobacco etch potyvirus infection

Project description:We have used a strain of Tobacco etch potyvirus (TEV) experimentally adapted to Arabidopsis thaliana ecotype Ler-0 to infect a set of seven A. thaliana plant ecotypes(Col-0, Ei-2, Wt-1, ler-0, Oy-0, St-0). Each ecotype was inoculated with the same amount of the virus. Using commercial microarrays containing probes Arabidopsis thaliana ssp. Col-0 plant transcripts, we explored the effect of viral infection in the plant transcriptome

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