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Caenorhabditis elegans

ABSTRACT: RNA-seq analysis of hsf-1 mutant in C. elegans larval development

PROVIDER: PRJNA321851 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR3535780.fastq.gz	Fastqsanger.gz
	SRR3535790.fastq.gz	Fastqsanger.gz
	SRR3535781.fastq.gz	Fastqsanger.gz
	SRR3535791.fastq.gz	Fastqsanger.gz
	SRR3535782.fastq.gz	Fastqsanger.gz

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Caenorhabditis elegans

Project description:RNA-seq analysis in C. elegans larval development and heat shock

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Caenorhabditis elegans

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RNA-seq analysis in C. elegans larval development and heat shock

Project description:To understand the function and regulation of the C. elegans heat shock factor (HSF-1) in larval development, we have used ChIP-seq to analyze the occupancy of HSF1 and RNA Pol II in L2 larvae and young adult (YA) animals grown at 20°C or upon heat shock at 34°C for 30 min. In addition, we have used RNA-seq to analyze the transcriptomes of wild type (N2), hsf-1(ok600) mutants and hsf-1(ok600); rmSi1[hsf-1::gfp] L2 larvae grown at 20°C and characterized the gene expression change by heat shock in wild type (N2), hsf-1(sy441) and hsf-1(sy441);rmSi1[hsf-1::gfp] animals at L2 stage.

2016-10-12 | GSE81520 | GEO

RNA-seq analysis of hsf-1 mutant in C. elegans larval development

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ChIP-seq of HSF-1 and Pol II in C. elegans larval development and heat shock

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Project description:Extensive oscillatory gene expression during C. elegans larval development [RNA-seq for polyA enriched mRNAs]

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Project description:The nematode Caenorhabditis elegans (C. elegans) is often used as a model organism to study cell and developmental biology. Quantitative mass spectrometry has only recently been performed in C. elegans and, so far, most studies have been done on adult worm samples. Here we use quantitative mass spectrometry to characterise protein level changes across the four larval developmental stages (L1-L4) of C. elegans, in biological triplicate. In total, we identify 4,130 proteins and quantify 1,541 proteins that were identified across all four stages in all three biological repeats with at least 2 unique peptides per protein. Using hierarchical clustering and functional ontological analyses, we identify 21 protein groups containing proteins with similar protein profiles across the four stages, and highlight the most overrepresented biological functions in each of these protein clusters. In addition, we use the dataset to identify putative larval stage specific proteins in each individual developmental stage, as well as in the early and late developmental stages. In summary, this dataset provides a system-wide analysis of protein level changes across the four C. elegans larval developmental stages, which serves as a useful resource for the worm development research community.

2017-12-04 | PXD006676 | Pride

Caenorhabditis elegans

Project description:Extensive oscillatory gene expression during C. elegans larval development [Ribosome footprinting]

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Caenorhabditis elegans

Project description:microRNA decay analysis in the first larval stage Caenorhabditis elegans

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Age-dependent heat shock hormesis to HSF-1 deficiency suggests a compensatory mechanism mediated by the unfolded protein response and innate immunity in young Caenorhabditis elegans

Project description:The goal of this RNA-Seq analysis was to identify genes differentially expressed in wild type (N2) and a hypomorphic mutant of the gene encoding heat shock factor 1 {hsf-1(sy441)} at normal conditions and upon heat shock. This study aimed to identify genes that are up- or downregulated when HSF-1 activity is impaired at 20 ºC. We also aimed to identify gene that are regulated by HSF-1 upon heat stress by comparing differentially expressed genes upon heat shock in wild type and in hsf-1(sy441) mutant background.

2024-06-29 | GSE241011 | GEO

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