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Mus musculus

ABSTRACT: RNA profiling of testis from wild-type and tamoxifen-induced NIPP1 knockout mice

PROVIDER: PRJNA325121 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR3655000_1.fastq.gz	Fastqsanger.gz
	SRR3655000_2.fastq.gz	Fastqsanger.gz
	SRR3655001_1.fastq.gz	Fastqsanger.gz
	SRR3655001_2.fastq.gz	Fastqsanger.gz
	SRR3655002_1.fastq.gz	Fastqsanger.gz

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RNA profiling of testis from wild-type and tamoxifen-induced NIPP1 knockout mice

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Proteome analysis of GCN1 CKO mouse liver

Project description:Tamoxifen-inducible conditional knockout (CKO) mice were generated to explore the function of Gcn1 in adult mice using the Cre/loxP system. To analyze the function of GCN1 in the liver, we compared the whole cell proteome of livers harvested from GCN1 CKO mice with that of wild-type mice.

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Proteome analysis of GCN1 CKO mouse ileum

Project description:Tamoxifen-inducible conditional knockout (CKO) mice were generated to explore the function of Gcn1 in adult mice using the Cre/loxP system. To analyze the function of GCN1 in the intestinal epithelium, we compared the whole cell proteome of ileum harvested from GCN1 CKO mice with that of wild-type mice.

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Project description:Spermatogenesis plays an important role in the mammalian testis, involving in the complex processes of mitosis, meiosis, and spermiogenesis. Spermatogenesis may also be disrupted in the absence of the immunological and ‘fence’ functions of the BTB, resulting in male subfertility or infertility. Mice lacking wild-type p53-induced phosphatase 1 (Wip1) display male reproductive organ defects, but the molecular mechanisms underlying these abnormalities remain unclear. We explored the function of Wip1 in spermatogenesis and fertility by examining differences in the expressed testis proteome and phosphoproteome between Wip1-deficient and wild-type mice using a proteomics approach. 90 proteins and 178 phosphoproteins were differentially regulated between these two groups of mice. These results suggested that proinflammatory cytokines may impair the blood–testis barrier dynamics by decreasing the expression of junction-associated proteins, which effect could be partially responsible for the subfertility and spermatogenesis defects in Wip1-knockout mice.

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