Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Proteome analysis of GCN1 CKO mouse liver

ABSTRACT: Tamoxifen-inducible conditional knockout (CKO) mice were generated to explore the function of Gcn1 in adult mice using the Cre/loxP system. To analyze the function of GCN1 in the liver, we compared the whole cell proteome of livers harvested from GCN1 CKO mice with that of wild-type mice.

ORGANISM(S): Mus Musculus (mouse)

SUBMITTER: Ken Itoh

PROVIDER: PXD030514 | JPOST Repository | Sat Apr 01 00:00:00 BST 2023

REPOSITORIES: jPOST

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	210625_M1083_IDA.wiff	Wiff
	210625_M1083_SWATH.wiff	Wiff
	210625_M1084_IDA.wiff	Wiff
	210625_M1084_SWATH.wiff	Wiff
	210625_M1084_SWATH.wiff.scan	Wiff

Items per page:

1 - 5 of 20

Publications

Inducible Systemic Gcn1 Deletion in Mice Leads to Transient Body Weight Loss upon Tamoxifen Treatment Associated with Decrease of Fat and Liver Glycogen Storage.

Liu Jun J Kasai Shuya S Tatara Yota Y Yamazaki Hiromi H Mimura Junsei J Mizuno Seiya S Sugiyama Fumihiro F Takahashi Satoru S Sato Tsubasa T Ozaki Taku T Tanji Kunikazu K Wakabayashi Koichi K Maeda Hayato H Mizukami Hiroki H Shinkai Yasuhiro Y Kumagai Yoshito Y Tomita Hirofumi H Itoh Ken K

International journal of molecular sciences 20220316 6

GCN1 is an evolutionarily-conserved ribosome-binding protein that mediates the amino acid starvation response as well as the ribotoxic stress response. We previously demonstrated that Gcn1 mutant mice lacking the GCN2-binding domain suffer from growth retardation and postnatal lethality via GCN2-independent mechanisms, while Gcn1-null mice die early in embryonic development. In this study, we explored the role of GCN1 in adult mice by generating tamoxifen-inducible conditional knoc ...[more]

PMID: 35328622

Similar Datasets

Proteome analysis of GCN1 CKO mouse jejunum

Project description:Tamoxifen-inducible conditional knockout (CKO) mice were generated to explore the function of Gcn1 in adult mice using the Cre/loxP system. To analyze the function of GCN1 in the intestinal epithelium, we compared the whole cell proteome of jejunum harvested from GCN1 CKO mice with that of wild-type mice.

2022-08-29 | PXD030519 | JPOST Repository

Proteome analysis of GCN1 CKO mouse duodenum

Project description:Tamoxifen-inducible conditional knockout (CKO) mice were generated to explore the function of Gcn1 in adult mice using the Cre/loxP system. To analyze the function of GCN1 in the intestinal epithelium, we compared the whole cell proteome of duodenum harvested from GCN1 CKO mice with that of wild-type mice.

2024-03-31 | PXD030517 | JPOST Repository

Proteome analysis of GCN1 CKO mouse ileum

Project description:Tamoxifen-inducible conditional knockout (CKO) mice were generated to explore the function of Gcn1 in adult mice using the Cre/loxP system. To analyze the function of GCN1 in the intestinal epithelium, we compared the whole cell proteome of ileum harvested from GCN1 CKO mice with that of wild-type mice.

2024-03-31 | PXD030518 | JPOST Repository

Proteome analysis of GCN1 CKO mouse inguinal white adipose tissue (iWAT)

Project description:Tamoxifen-inducible conditional knockout (CKO) mice were generated to explore the function of Gcn1 in adult mice using the Cre/loxP system. To analyze the function of GCN1 in the inguinal white adipose tissue (iWAT), we compared the whole cell proteome of eWAT harvested from GCN1 CKO mice with that of wild-type mice.

2024-03-31 | PXD030516 | JPOST Repository

Decoupling astrocytes in adult mice impairs synaptic plasticity and spatial learning

Project description:To study the role of the gap junction coupled astrocytic network, we generated inducible double knockouts to selectively delete Cx30 and Cx43 from astrocytes in adult mice. Mice carrying loxP-flanked Gjb6 (Cx30fl/fl mice) (Boulay et al., 2013) and Gja1 (Cx43fl/fl mice) (Theis et al., 2003) alleles were crossbred with mice expressing the tamoxifen-sensitive Cre-recombinase CreERT2 under the endogenous GLAST(Slc1a3)-promoter (Mori et al., 2006). Adult, 8-10 week old mice (Cx30fl/fl:Cx43fl/fl:GLASTCreERT2/+, termed cKO) and littermate control mice (Cx30fl/fl:Cx43fl/fl:GLAST+/+) were injected with tamoxifen for 5 consecutive days and hippocampi were isolated for subsequent TMT-based proteomics analysis 90 days after tamoxifen treatment, to study the consequences of astrocyte decoupling and connexin deletion.

2022-03-14 | PXD031136 | Pride

Next Generation Sequencing of Loxp (Rxratm1Krc/J,Rxra loxP), Hetero(Nex-cre;Rxratm1Krc/J,Rxra cKO-Het) and cKO (Nex-cre;Rxratm1Krc/J,Rxra cKO) mouse Transcriptomes

Project description:When compared with Loxp, both Hetero and cKO mice showed reduced expression of immediate early genes

2019-09-04 | GSE136788 | GEO

Genome-wide analysis of gene expression of Flk1Cre;ETV2 CKO mouse yolk sacs.

Project description:To identify genes critical for vascular development, we generated mice where ETV2 is inactivated in FLK1+ cells by a loxP-Cre recombination approach. Results provide a detailed insight into the function of ETV2 in emrbyonic vasculare formation. Total RNA obtained from E9.5 yolk sacs from Flk1Cre;ETV2 CKO and control mice.

2014-11-17 | E-GEOD-51407 | biostudies-arrayexpress

Mechanisms of stop codon readthrough mitigation reveal principles of GCN1 mediated translational quality control

Project description:Readthrough into the 3′ untranslated region (3′UTR) of the mRNA results in the production of aberrant proteins. Metazoans efficiently clear readthrough proteins, but the underlying mechanisms remain unknown. Here, we show in C. elegans and mammalian cells that readthrough proteins are targeted by a coupled, two-level quality control pathway involving the BAG6 chaperone complex and the ribosome collision-sensing protein GCN1. Readthrough proteins with hydrophobic C-terminal extensions are recognized by SGTA-BAG6 and ubiquitylated by RNF126 for proteasomal degradation. Additionally, cotranslational mRNA decay initiated by GCN1 and CCR4/NOT limits the accumulation of readthrough products. Unexpectedly, selective ribosome profiling uncovered a general role of GCN1 in regulating translation dynamics when ribosomes encounter nonoptimal codons, a feature of 3′UTR sequences. Dysfunction of GCN1 results in mRNA and proteome imbalance, increasingly perturbing transmembrane proteins and collagens during aging. These results define GCN1 as a key factor acting during translation in maintaining protein homeostasis.

2023-05-15 | GSE214396 | GEO

Bulk RNASeq of mouse astrocytes isolated from the somatosensory cortex of control and Tcf7l2 conditional knockout (TdTomato+).

Project description:The aim of the experiment was to explain the role of astrocytic TCF7L2 transcription factor in astrocyte development and maturation. To do that we used Tcf7l2FL/FL:TdTomatoFL/FL animals with loxP sites that flanked exon 6, the deletion of which causes a frameshift mutation and leads to nonsense-mediated decay. Those mice were crossed with Aldh1l1Cre-ERT2 animals, that carried a transgene with tamoxifen-inducible Cre recombinase under the control of an astrocyte lineage-specific promoter. Control line was obtained crossing Aldh1l1Cre-ERT2 with Tcf7l2WT/WT:TdTomatoFL/FL animals. Tamoxifen (75 mg/kg body weight) was administered on postnatal day 6 and 8, 27 days later, (postnatal day 35), mice were sacrificed and TdT postive astrocytes were FACS-sorted using BD FACSAria, RNA from 4 control TdT and 3 Tcf7l2 cKO TdT mice were isolated and bulk RNA-seq was performed.

2024-06-28 | E-MTAB-13153 | biostudies-arrayexpress

Regulation of the cardiac transcriptome by the E3 ubiquitin ligase Huwe1.

Project description:Analysis of the cardiac transcriptome by heart-specific acute genetic ablation of Huwe1 in adult male mice employing a tamoxifen-inducible Cre-loxP system.

2016-09-30 | E-MTAB-5112 | biostudies-arrayexpress