Project description:Plant-activator is known as a chemical inducer to activate disease resistances in plants without direct antibacterial activities. To identify plant-activator responsive genes, we analyzed temporal expression changes of the genes when treated with some plant-activators (e.g., isotianil and probenazole) in Japonica rice cv. Nipponbare in a field at Tsukuba in Japan.
Project description:A continuous gene expression profiling of leaves was performed at regular interval during the sunset period to understand the changes of transcriptional program in the rice plant grown under natural field conditions in response to solar radiation. At 78 days after transplanting, leaf samples corresponding to the uppermost fully expanded leaves were collected at 10-min intervals from 5:00 PM until 8:00 PM. All samples were obtained from rice plants grown in the field during the 2008 cultivation season.
Project description:A continuous gene expression profiling of leaves was performed at regular interval during the sunrise period to understand the changes of transcriptional program in the rice plant grown under natural field conditions in response to solar radiation. At 79 days after transplanting, leaf samples corresponding to the uppermost fully expanded leaves were collected at 10-min intervals in two replicates from 3:50 AM until 6:00 AM. All samples were obtained from rice plants grown in the field during the 2008 cultivation season.
Project description:The experiment tested organ-specific responses of rice (Oryza sativa ssp. japonica) to cold stress with a special focus on phytohormonal regulation. Cold stress (5°C, 24 h) was applies on the whole plants, leaves or roots. The results showed distinct responses when cold stress was applied on leaves, relating to photosynthesis and sugar synthesis as well as specific changes in phytohormones. On the other hand, stress applied to roots was more similar to the stress on the whole plant indicating roots to be more important in cold stress responses. Acclimation by mild temperature (15°C, 12 h) highlighted changes which are connected even with lower temperature exposure or which are characteristic for untreated organs. Recovery (3 d) indicated ability of plants to restore growth which correlated between individual phytohormones and plant growth. The article connect transcriptome, hormonome, proteome and sugar analyses of rice cold-stress responses.
Project description:A continuous gene expression profiling of leaves at regular interval was performed to understand the changes of transcriptional program and physiological state of the rice plant under two different field conditions.
Project description:Benzothiadiazole (BTH) is a so-called âplant activatorâ and protects plants from diseases by activating the salicylic-acid (SA) signaling pathway. We identified BTH-responsive genes in rice leaves 24 h after treatment using rice 44K microarray. Keywords: response to chemical treatment BTH-treated leaves were compared with mock-treated leaves using single-color method with 4 biological replicates.
Project description:Ghd7 regulates a range of functions in growth and development in response to environmental cues to maximize the reproductive success of the rice plant. We used microarrays to detail the regulated genes in OX-Ghd7HJ19 transgenic plants. To gain clues to downstream genes regulated by Ghd7, we performed a microarray analysis using Affimetrix rice gene chips. Young leaves in vegetative stage (35-days old) and developing panicles (0.1cm) from field-grown OX-Ghd7HJ19 transgenic and wild-type plants with two biological replicates were used to isolate RNA for chip analysis.
Project description:A continuous gene expression profiling of leaves was performed during nighttime at regular interval from transplanting until harvesting to understand the changes of transcriptional program and physiological state throughout entire growth of the rice plant under natural field conditions. The uppermost fully-expanded leaf in the main stem corresponding to the 1st leaf until 83 days after transplanting (DAT) and the flag leaf from them on were sampled in two replicates at 12:00 midnight at 7- day intervals encompassing 17 different growth stages. All samples were obtained from rice plants grown in the field during the 2008 cultivation season.
