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Drosophila melanogaster

ABSTRACT: Chromatin Binding of Gcn5 in Drosophila is Largely Mediated by CP190 [RNA-seq]

PROVIDER: PRJNA325818 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR3671204.fastq.gz	Fastqsanger.gz
	SRR3671205.fastq.gz	Fastqsanger.gz
	SRR3671206.fastq.gz	Fastqsanger.gz
	SRR3671207.fastq.gz	Fastqsanger.gz
	SRR3671208.fastq.gz	Fastqsanger.gz

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Drosophila melanogaster

Project description:Chromatin Binding of Gcn5 in Drosophila is Largely Mediated by CP190 [ChIP-seq]

| PRJNA325817 | ENA

Chromatin Binding of Gcn5 in Drosophila is Largely Mediated by CP190

Project description:Chromatin Binding of Gcn5 in Drosophila is Largely Mediated by CP190

| PRJNA325821 | ENA

Chromatin Binding of Gcn5 in Drosophila is Largely Mediated by CP190 [ChIP-seq]

Project description:80% of the genomic binding sites of the histone acetyltransferase Gcn5 are colocalizing with CP190 binding. Depletion of CP190 reduces the number of Gcn5 binding sites and binding strength to chromatin. Binding dependency was further supported by Gcn5 mediated co-precipitation of CP190

2016-12-05 | GSE83408 | GEO

Chromatin Binding of Gcn5 in Drosophila is Largely Mediated by CP190 [RNA-seq]

2016-12-05 | GSE83406 | GEO

CTCF is required to form regulatory and physical boundaries at limited sites in the Drosophila nervous system [ChIP-seq]

Project description:CCCTC-binding factor (CTCF) is a conserved protein able to block communication between regulatory elements and gene promoters in flies and mammals. Here, we studied CTCF function in the Drosophila central nervous system (CNS) in which we find CTCF plays a vital role. Chromatin immunoprecipitation (ChIP)-sequencing of CTCF and its partner protein Centrosomal protein 190 kDa (Cp190) in CNSs of wildtype (WT), CTCF[0] mutants completely lacking CTCF and Cp190[KO] mutants with a CRISPR/Cas9-mediated deletion of the Cp190 open reading frame identified CTCF and Cp190 peaks defined as enriched in WT relative to each respective mutant control. These experiments also revealed that CTCF recruits Cp190 to CTCF's binding sites, but Cp190 is dispensable for CTCF binding to most of CTCF's sites.

2021-01-18 | GSE146749 | GEO

Mechanism and functional role of the interaction between CP190 and the architectural protein Pita in Drosophila melanogaster

Project description:The architectural protein Pita is critical for Drosophila embryogenesis and predominantly binds to gene promoters and insulators. In particular, Pita is involved in the organization of boundaries between regulatory domains, controlled by the expression of three hox genes in the Bithorax complex (BX-C). The best-characterized partner for Pita is the BTB/POZ-domain containing protein CP190. Here, we precisely mapped two unstructured regions of Pita that interact with the BTB domain of CP190. The deletion of the CP190-interacting regions did not significantly affect the binding of the mutant Pita protein to most chromatin sites. The expression of the mutant protein completely complemented the null pita mutation. However, the mutant Pita protein does not support the ability of multimerized Pita sites to prevent cross-talk between the iab-6 and iab-7 regulatory domains that activate the expression of Abdominal-B (Abd-B), one of the genes in the BX-C. The recruitment of the Pita region and the interaction with CP190 and the polytene chromosomes of larvae induces the formation of a new interband, which is a consequence of the formation of open chromatin in this region. These results suggested that the interaction with CP190 is required for the primary Pita activities, but other architectural proteins may also recruit CP190 in flies expressing only the mutant Pita protein.

2020-11-30 | GSE160007 | GEO

Ibf1 and Ibf2 are DNA-binding proteins required for insulator function in Drosophila [ChIP-seq]

Project description:Here we report on the identification of two previously uncharacterized proteins as CP190 interacting proteins, that we have named Ibf1 and Ibf2. These proteins localize at insulator bodies and associate with chromatin at CP190-binding sites throughout the genome. We also show that Ibf1 and Ibf2 are DNAbinding proteins that form obligated hetero-oligomers that mediate CP190 binding to chromatin. Moreover, Ibf1 and Ibf2 are necessary for insulator activity in enhancerblocking assays. Taken together our data reveal a novel pathway of CP190 recruitment to chromatin that is required for insulator activity. ChIP-Seq peak calling of CP190, Ibf1 and Ibf2 against Input sample in Drosophila melanogaster S2 cells

2014-04-18 | E-GEOD-46614 | biostudies-arrayexpress

Drosophila melanogaster

Project description:Gene expression analysis of Drosophila melanogaster S2 cells after RNAi-mediated depletion of the insulator factor CP190

| PRJNA114125 | ENA

Drosophila melanogaster

Project description:Ibf contributes to CP190 recruitment to chromatin [ChIP-seq]

| PRJNA227235 | ENA

CTCF is required to form regulatory and physical boundaries at limited sites in the Drosophila nervous system [RNA-seq]

Project description:CCCTC-binding factor (CTCF) is a conserved protein able to block communication between regulatory elements and gene promoters in flies and mammals. Here, we studied CTCF function in the Drosophila central nervous system (CNS) in which we find CTCF plays a vital role. RNA-sequencing of CNSs of CTCF[0] mutants that completely lack CTCF identified putative targets that are expressed in altered patterns. We find that CTCF recruits Centrosomal protein 190 kDa (Cp190) to CTCF's binding sites to co-regulate a substantial fraction of common genes that we find are also altered in CNSs of Cp190[KO] mutants carrying a CRISPR/Cas9-mediated deletion of the Cp190 open reading frame.

2021-01-18 | GSE146751 | GEO

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