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Chromatin Binding of Gcn5 in Drosophila is Largely Mediated by CP190

ABSTRACT: Chromatin Binding of Gcn5 in Drosophila is Largely Mediated by CP190

PROVIDER: PRJNA325821 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR3671204.fastq.gz	Fastqsanger.gz
	SRR3671294.fastq.gz	Fastqsanger.gz
	SRR3671205.fastq.gz	Fastqsanger.gz
	SRR3671295.fastq.gz	Fastqsanger.gz
	SRR3671206.fastq.gz	Fastqsanger.gz

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Chromatin Binding of Gcn5 in Drosophila is Largely Mediated by CP190 [ChIP-seq]

Project description:80% of the genomic binding sites of the histone acetyltransferase Gcn5 are colocalizing with CP190 binding. Depletion of CP190 reduces the number of Gcn5 binding sites and binding strength to chromatin. Binding dependency was further supported by Gcn5 mediated co-precipitation of CP190

2016-12-05 | GSE83408 | GEO

Chromatin Binding of Gcn5 in Drosophila is Largely Mediated by CP190 [RNA-seq]

2016-12-05 | GSE83406 | GEO

Chromatin binding of Gcn5 in Drosophila is largely mediated by CP190.

Project description:Centrosomal 190 kDa protein (CP190) is a promoter binding factor, mediates long-range interactions in the context of enhancer-promoter contacts and in chromosomal domain formation. All Drosophila insulator proteins bind CP190 suggesting a crucial role in insulator function. CP190 has major effects on chromatin, such as depletion of nucleosomes, high nucleosomal turnover and prevention of heterochromatin expansion. Here, we searched for enzymes, which might be involved in CP190 mediated chromatin changes. Eighty percent of the genomic binding sites of the histone acetyltransferase Gcn5 are colocalizing with CP190 binding. Depletion of CP190 reduces Gcn5 binding to chromatin. Binding dependency was further supported by Gcn5 mediated co-precipitation of CP190. Gcn5 is known to activate transcription by histone acetylation. We used the dCas9 system to target CP190 or Gcn5 to a Polycomb repressed and H3K27me3 marked gene locus. Both, CP190 as well as Gcn5, activate this locus, thus supporting the model that CP190 recruits Gcn5 and thereby activates chromatin.

| S-EPMC5389606 | biostudies-literature

Chromatin Binding of Gcn5 in Drosophila is Largely Mediated by CP190

Project description:This SuperSeries is composed of the SubSeries listed below.

2016-12-05 | GSE83409 | GEO

Drosophila melanogaster

Project description:Chromatin Binding of Gcn5 in Drosophila is Largely Mediated by CP190 [ChIP-seq]

| PRJNA325817 | ENA

Drosophila melanogaster

Project description:Chromatin Binding of Gcn5 in Drosophila is Largely Mediated by CP190 [RNA-seq]

| PRJNA325818 | ENA

Topological screen identifies hundreds of Cp190 and CTCF dependent Drosophila chromatin insulator elements [HiC]

Project description:CTCF and Cp190 proteins are implicated at many insulator elements throughout Drosophila genome. Here we compared Hi-C maps, transcriptomes and binding of multiple insulator proteins in cultured Drosophila cells derived from CTCF-KO, Cp190-KO and control embryos.

2022-07-19 | GSE198760 | GEO

Topological screen identifies hundreds of Cp190 and CTCF dependent Drosophila chromatin insulator elements [ChIP-seq]

2022-07-19 | GSE198762 | GEO

Topological screen identifies hundreds of Cp190 and CTCF dependent Drosophila chromatin insulator elements [RNA-seq]

2022-07-19 | GSE198761 | GEO

M1BP cooperates with CP190 to activate transcription at TAD borders and promote chromatin insulator activity

Project description:Genome organization is driven by forces affecting transcriptional state, but the relationship between transcription and genome architecture remains unclear. Here, we identified the Drosophila transcription factor Motif 1 Binding Protein (M1BP) in physical association with the gypsy chromatin insulator core complex, including the universal insulator protein CP190. M1BP is required for enhancer-blocking and barrier activities of the gypsy insulator as well as its proper nuclear localization. Genome-wide, M1BP specifically colocalizes with CP190 at Motif 1-containing promoters, which are enriched at topologically associating domain (TAD) borders. M1BP facilitates CP190 chromatin binding at many shared sites and vice versa. Both factors promote Motif 1-dependent gene expression and transcription near TAD borders genome-wide. Finally, loss of M1BP reduces chromatin accessibility and increases both inter- and intra-TAD local genome compaction. Our results reveal physical and functional interaction between CP190 and M1BP to activate transcription at TAD borders and mediate chromatin insulator-dependent genome organization.

2021-06-07 | PXD026497 | Pride

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