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Gestational choline supplementation improves cross-generational mood by epigenetic upregulation of Nr3c1

ABSTRACT: Gestational choline supplementation improves cross-generational mood by epigenetic upregulation of Nr3c1

PROVIDER: PRJNA328805 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR3880162_1.fastq.gz	Fastqsanger.gz
	SRR3880162_2.fastq.gz	Fastqsanger.gz
	SRR3880163_1.fastq.gz	Fastqsanger.gz
	SRR3880163_2.fastq.gz	Fastqsanger.gz
	SRR3880164_1.fastq.gz	Fastqsanger.gz

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Gestational choline supplementation improves cross-generational mood by epigenetic upregulation of Nr3c1 [RRBS]

Project description:Environmental cues during gestation could exert transgenerational effects on behavior mainly through changes in epigenetic marks such as DNA methylation. However, the role of histone modifications in the inheritance of acquired phenotypes remains largely unknown in mammals. Here, we report that gestational choline supplementation (GCS) in maternal mice exerts anxiolytic effects on male offspring across 2 generations. Correspondingly, GCS-induced H3K9 hyperacetylation in the Nr3c1 promoter in male hippocampus leads to upregulation of Nr3c1 and its encoded protein, glucocorticoid receptor (GR), across 2 generations through the male germline. Furthermore, inhibition of CREB-binding protein (CBP) histone acetyltransferase (HAT) function restored GCS-induced epigenetic and behavioral alterations, suggesting that CBP function as a HAT to increase Nr3c1 expression through H3K9 hyperacetylation. Thus, GCS-induced GR upregulation through CBP-mediated H3K9 hyperacetylation in the Nr3c1 promoter is associated with anxiolytic behavior in male offspring, highlighting the role of histone modification in acquired phenotypes across mammalian generations.

2021-09-01 | GSE84316 | GEO

Gestational choline supplementation improves cross-generational mood by epigenetic upregulation of Nr3c1 [RNA-Seq]

2021-09-01 | GSE84315 | GEO

Gestational choline supplementation improves cross-generational mood by epigenetic upregulation of Nr3c1 [ChIP-Seq]

2021-09-01 | GSE84314 | GEO

Gestational choline supplementation improves cross-generational mood by epigenetic upregulation of Nr3c1

Project description:This SuperSeries is composed of the SubSeries listed below.

2021-09-01 | GSE84317 | GEO

Mus musculus

Project description:Gestational choline supplementation improves cross-generational mood by epigenetic upregulation of Nr3c1 [RRBS]

| PRJNA328811 | ENA

Mus musculus

Project description:Gestational choline supplementation improves cross-generational mood by epigenetic upregulation of Nr3c1 [RNA-Seq]

| PRJNA328812 | ENA

Mus musculus

Project description:Gestational choline supplementation improves cross-generational mood by epigenetic upregulation of Nr3c1 [ChIP-Seq]

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Modulation of liver cholesterol homeostasis by choline supplementation during fibrosis resolution

Project description:Liver fibrosis is a critical global health challenge, often leading to severe liver diseases without timely intervention. Choline deficiency has been linked to metabolic dysfunction associated steatohepatitis (MASH) and liver fibrosis, suggesting choline supplementation as a potential therapeutic approach. This study aimed to explore the therapeutic potential of choline supplementation in liver fibrosis resolution and its effects on cholesterol homeostasis using a mouse model with induced liver fibrosis. Our findings reveal that choline supplementation significantly decreases blood lactate dehydrogenase (LDH) and non-high-density lipoprotein cholesterol (non-HDL-C) levels. Transcriptome analysis showed that choline supplementation primarily induces genes related to cholesterol homeostasis, suggesting a significant impact on liver cholesterol synthesis. However, choline supplementation did not significantly alter the expression of fibrosis-related, choline metabolism-related, or epigenetics-related genes. This study provides novel insights into the role of choline in liver health and cholesterol metabolism, potentially informing treatments for liver fibrosis and related conditions.

2024-09-04 | GSE261310 | GEO

Transcriptional profiling of mouse liver tissue following control, choline and methonine/choline deficient diet intervention

Project description:Choline and methionine/choline deficient diets are widely used to generate severe rodent hepatic steatosis and steatohepatitis in an attempt to reflect stages of human non-alcoholic fatty liver disease (NAFLD). The underlying mechanism of hepatic injury in these models, and how this reflects human disease remains incompletely understood. We used detailed transcriptional analysis to interrogate the molecular mechanisms of this intervention and its similarity to human disease. Adult C57Bl/6J mice were maintained on control, choline deficient (CDD) or methionine/choline deficient (MCDD) diets for 4 weeks. Isolated liver RNA was used for transcriptional profiling by micro array analysis.

2016-06-01 | E-MTAB-3943 | biostudies-arrayexpress

Manipulation of NR3C1 on the expression of inflammatory transcription factors and their alternative splicing patterns

Project description:NR3C1-encoding glucocorticoid receptors have dual roles as RNA-binding protein and transcription factor. Recent studies revealed that NR3C1 might play an important role in the pathogenesis of PTSD (Post-traumatic stress disorder). However, its molecular mechanism remained unclear. In the present study, a neuronal cell model was constructed by transfecting a NR3C1-overexpressing plasmid pIRES-hrGFP-1a-NR3C1 or empty vector into HT22 cells. The changes in global transcription levels and alternative splicing events in HT22 cells after NR3C1 overexpression were analyzed by RNA sequencing. Compared with the empty vector control, the expression of inflammatory factors were differentially regulated by NR3C1, including genes involved in chemokine signal pathway, PI3K-Akt signal pathway, cytokine receptor interaction, neural ligand-receptor interaction and so on. In addition, NR3C1 regulated the alternative splicing of many genes involved in immune response, axon formation, stress response and inflammation. This study was the first to perform a transcriptome analysis of differential gene expression and alternative splicing in a NR3C1-overexpressing HT22 cell model. Our results suggested that NR3C1 could manipulate the expression of inflammatory transcription factors and their alternative splicing patterns, subsequently affecting the expression of downstream targets, leading to the onset of PTSD. This study will provide new insights in the NR3C1-mediated gene regulation in relation to PTSD.

2024-11-26 | GSE189587 | GEO

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