Project description:The objective of this study is to investigate the protein expression level difference between Mycobacterium avium hominissuis wild type strain MAH-104 , its mutant 21m (gene lysX (MAV_3128) mutated - Lysyl tRNA synthetase) and the complemented strain 21c (MAV_3128comp) using liquid chromatography-mass spectrometry (LC/MS) based label free quantitative proteomics analysis.
Project description:An opportunistic intracellular pathogen Mycobacterium avium subsp. hominissuis, a member of the nontuberculous mycobacteria (NTM) cluster, causes respiratory disease in immunosuppressed hosts. In particular, infected companion dogs are a potential role to transmit the agent to children or immunosuppressed people. The purpose of this study is to investigate a host-M. avium hominissuis interactome in canine PBMCs during the infection.