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Small RNAs produced in the wild-type and mutant Dicer-2 transgenic flies

ABSTRACT: small RNAs produced in the wild-type and mutant Dicer-2 transgenic flies

OTHER RELATED OMICS DATASETS IN: GSE84532

PROVIDER: PRJNA329574 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR3929730.fastq.gz	Fastqsanger.gz
	SRR3929731.fastq.gz	Fastqsanger.gz
	SRR3929732.fastq.gz	Fastqsanger.gz
	SRR3929723.fastq.gz	Fastqsanger.gz
	SRR3929733.fastq.gz	Fastqsanger.gz

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small RNAs produced in the mutant Dicer-2 transgenic flies

Project description:small RNAs produced in the mutant Dicer-2 transgenic flies

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small RNAs produced in the mutant Dicer-2 transgenic flies

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small RNAs produced in the wild-type and mutant Dicer-2 transgenic flies

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Project description:Drosophila Dicer-1 produces microRNAs (miRNAs) from pre-miRNA, whereas Dicer-2 generates small interfering RNAs (siRNAs) from long dsRNA. loquacious (loqs) encodes three Dicer partner proteins, Loqs-PA, Loqs-PB, and, Loqs-PD, generated by alternative splicing. To understand the function of each Loqs isoform, we constructed loqs isoform-specific rescue flies. Loqs-PD promotes siRNA production in vivo by Dicer-2. Loqs-PA or Loqs-PB is required for viability, but the proteins are not fully redundant: Loqs-PB is required to produce a specific subset of miRNAs. Surprisingly, Loqs-PB tunes the product size cleaved by Dicer-1 from pre-miR-307a, generating a longer miRNA isoform with a distinct seed sequence and target specificity. The mouse and human Dicer-binding partner TRBP, a homolog of Loqs-PB, similarly tunes the site of pre-miR-132 cleavage by mammalian Dicer. Thus, Dicer-binding partner proteins can change the choice of cleavage site by Dicer, producing miRNAs with different target specificities than those that would be made by Dicer alone.

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