Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Saccharomyces cerevisiae

ABSTRACT: Spo11-oligo mapping in S. cerevisiae red1, hop1, mek1 mutants

PROVIDER: PRJNA335382 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR3953720_1.fastq.gz	Fastqsanger.gz
	SRR3953720_2.fastq.gz	Fastqsanger.gz
	SRR3953721_1.fastq.gz	Fastqsanger.gz
	SRR3953721_2.fastq.gz	Fastqsanger.gz
	SRR3953722_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 12

Similar Datasets

Spo11-oligo mapping in S. cerevisiae red1, hop1, mek1 mutants

Project description:Meiotic recombination starts with the formation of DNA double-strand breaks (DSBs) made by Spo11. In Saccharomyces cerevisiae, the nonrandom distribution of meiotic DSBs along the genome can be attributed to the combined influence of multiple factors on Spo11 cleavage. One factor is higher-order chromatin structure, particularly the loop-axis organization of meiotic chromosomes. Axial element proteins Red1 and Hop1 provide the basis for meiotic loop-axis organization and are implicated in diverse aspects of meiotic recombination. Mek1 is a meiotic-specific kinase associated with Red1 and Hop1. Red1, Hop1, and Mek1 are required for normal DSB levels, but their effects on the DSB distribution has not been examined, and exactly how these proteins influence DSB levels and distribution is unknown. Here, we examined the contributions of Red1, Hop1, and Mek1 to the DSB distribution by deep sequencing and mapping Spo11-associated oligonucleotides from red1, hop1, and mek1 mutant strains, thereby generating genome-wide meiotic DSB maps.

2018-09-01 | GSE84859 | GEO

Saccharomyces cerevisiae

Project description:Spo11-oligo mapping in zip3 mutants

| PRJNA209592 | ENA

Saccharomyces cerevisiae

Project description:Spo11-oligo mapping in bas1 and ino4 mutants

| PRJNA281182 | ENA

Spo11-oligo mapping in S. cerevisiae strain SK1

Project description:Spo11-oligo mapping in S. cerevisiae strain SK1

2020-07-23 | GSE152957 | GEO

Saccharomyces cerevisiae

Project description:Spo11-oligo mapping in S. cerevisiae Ctf19/CCAN kinetochore sub-complex mutant mcm21

| PRJNA294661 | ENA

Turning coldspots into hotspots: targeted recruitment of axis protein Hop1 stimulates meiotic recombination in Saccharomyces cerevisiae

Project description:The DNA double strand breaks (DSBs) that initiate meiotic recombination are formed in the context of the meiotic chromosome axis, which in budding yeast contains a meiosis-specific cohesin isoform and the meiosis-specific proteins Hop1 and Red1. Hop1 and Red are important for DSB formation; DSB levels are reduced in their absence and their levels, which vary along the lengths of chromosomes, are positively correlated with DSB levels. How axis protein levels influence DSB formation and recombination remains unclear. To address this question, we developed a novel approach that uses a bacterial ParB-parS partition system to recruit axis proteins at high levels to inserts at recombination coldspots where Hop1 and Red1 levels are normally low. Recruiting Hop1 markedly increased DSBs and homologous recombination at target loci, to levels equivalent to those observed at endogenous recombination hotspots. This local increase in DSBs did not require Red1 or the meiosis-specific cohesin component Rec8, indicating that, of the axis proteins, Hop1 is sufficient to promote DSB formation. However, while most crossovers at endogenous recombination hotspots are formed by the meiosis-specific MutLγ resolvase, only a small fraction of crossovers that formed at an insert locus required MutLγ, regardless of whether or not Hop1 was recruited to that locus. Thus, while local Hop1 levels determine local DSB levels, the recombination pathways that repair these breaks can be determined by other factors, raising the intriguing possibility that different recombination pathways operate in different parts of the genome.

2022-07-12 | GSE201240 | GEO

Saccharomyces cerevisiae

Project description:Chromatin IP for Mcm2-7, Rec8, Hop1 and Red1

| PRJNA155861 | ENA

Spo11-oligo mapping in S. cerevisiae strain SK1

Project description:Spo11-oligo mapping in S. cerevisiae strain SK1

| PRJNA641078 | ENA

Saccharomyces cerevisiae

Project description:Spo11-oligo mapping of DSBs in an SK1 x S288C F1 hybrid strain

| PRJNA394003 | ENA

Spo11-oligo mapping in wild type S. cerevisiae strain

Project description:Spo11-oligo mapping in wild type S. cerevisiae strain