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ABSTRACT: IVT-SAPAS Library for VSV infection in macrophages

PROVIDER: PRJNA340928 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR4217160.fastq.gz	Fastqsanger.gz
	SRR4217170.fastq.gz	Fastqsanger.gz
	SRR4217161.fastq.gz	Fastqsanger.gz
	SRR4217171.fastq.gz	Fastqsanger.gz
	SRR4217162.fastq.gz	Fastqsanger.gz

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Expression data from mouse peritoneal macrophages

Project description:Biologic functions involved in innate immune response of macrophages rely on the precise regulation of kinds of immune molecular. In the virus infection procession, the macrophages are activated following a tightly controlled genetic programme where specific sets of genes are up-regulated or down-regulated. We used microarrays to detail the global programme of gene expression underlying VSV infection and identified distinct classes of up-regulated and down-regulated genes during this process. Mouse peritoneal macrophages were selected with/without VSV infection for 8 hours for RNA extraction and hybridization on Affymetrix microarrays. We sought to obtain expression profiles. We selected macrophages according to VSV infection at two time-points: uninfected macrophage(control) and VSV infected for 8 hour macrophages(VSV).

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Project description:IRF3 is one of the most critical transcription factor in down stream of pattern recognition receptors (such as toll-like receptor and RIG-I-like receptor) signalling pathway. IRF3 is known to induce the expression of type I IFN gene upon virus infection. To furter examine the role of IRF3 in virus-induced gene expression, we preformed microarray analysis in IRF3-/- peritoneal macrophages infected with VSV, and found that IRF3 suppresses the expression of Il12b gene. Peritoneal macrophages from WT of IRF3-/- B6 mice were infected with VSV(1 M.O.I. ) for 6 hous, and then subjected to microarray analysis.

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