Project description:Transcriptional profiling reveals differential changes in the respective expression of oxytocin and CART mRNA levels in the nucleus accumbens and dorsal striatum of compulsive methamphetamine taking rats (nucleus accumbens)
Project description:Methamphetamine (METH) is a powerful stimulant that has caused addiction (compulsive drug seeking and taking behavior) in millions of people world-wide. METH abuse is also associated with negative impact on the brain. One feature of addiction is uncontrollable drug seeking despite adverse consequences and becomes habitual. To mimic this in a rat model, rats with a history of METH use are given the opportunity to earn METH accompanied by aversive shocks on their feet. Rats that continue to take METH are shock-resistant (SR) and rats that reduce their METH intake are shock-sensitive (SS ).Rats that self-administered saline are controls (CT). Thereafter, rats were injected intraperitoneally with the dopamine D1 receptor antagonist, SCH23390. SCH23390 caused substantial reduction of METH taking in a dose-dependent fashion. Stopping SCH23390 administration led to re-emergence of compulsive METH taking in the shock-resistant rats.
Project description:Transcriptional profiling reveals differential changes in the expression of oxytocin and CARTpt mRNAs in the nucleus accumbens and dorsal striatum of rats (striatum)
Project description:Methamphetamine administration causes differential alterations in gene expression and dynamic patterns of histone acetylation/hypoacetylation in the rat nucleus accumbens
Project description:Transcriptomic analysis of prefrontal medial cortex, hippocampus and nucleus accumbens of alcohol-preferring and nonpreferring rats
Project description:Using ssRNA-seq, we examined the alteration of transcription profiles in the nucleus accumbens of methamphetamine-sensitized mice. Methamphetamine was a commonly abused psychostimulant. Repeated exposure to methamphetamine elicited long-lasting cellular and molecular changes, including the aberrant expression of coding and non-coding RNAs, which may involve in methamphetamine-induced locomotor sensitization and addiction.
