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Saccharomyces cerevisiae

ABSTRACT: A High Resolution Profile of NMD Substrates in Yeast

PROVIDER: PRJNA341846 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR4163290.fastq.gz	Fastqsanger.gz
	SRR4163300.fastq.gz	Fastqsanger.gz
	SRR4163291.fastq.gz	Fastqsanger.gz
	SRR4163301.fastq.gz	Fastqsanger.gz
	SRR4163292.fastq.gz	Fastqsanger.gz

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A High Resolution Profile of NMD Substrates in Yeast

Project description:We report a high resolution catalouge of NMD substrates using RNA-Seq. We discovered several hundred new substrates for NMD. Using published ribosome footprint profiling data, we measured ribosome densities of normal-looking NMD substrates and non-NMD substrates. NMD substrates exhibited a striking difference in normalized ribosome occupancy in wild-type and UPF1 cells. We also found that normal looking NMD substrates have higher ratio of out of frame reads, lower codon optimalites and a higher propensity to have long stretches of non-optimal codons.

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