Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Mus musculus

ABSTRACT: mRNA profiling of mouse embryonic cells with activin or SB431542 treatment

PROVIDER: PRJNA342470 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR4217430.fastq.gz	Fastqsanger.gz
	SRR4217431.fastq.gz	Fastqsanger.gz
	SRR4217432.fastq.gz	Fastqsanger.gz
	SRR4217433.fastq.gz	Fastqsanger.gz
	SRR4217434.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 15

Similar Datasets

mRNA profiling of mouse embryonic cells with activin or SB431542 treatment

Project description:We analysed the mRNA expression profiles of control and VprBP knockdown mouse embryonic stem cells (mESCs) with or without Activin treatment or TGF-β/Activin inhibitor SB431542 challenge for 4 days during differentiation

2016-09-10 | GSE86631 | GEO

Activin/Nodal signaling in mouse embryonic stem cells

Project description:Members of the transforming growth factor (TGF)-β superfamily play essential roles in the pluripotency, self-renewal, and differentiation of embryonic stem cells. While bone morphogenic proteins maintain pluripotency of undifferentiated mouse ES cells, the role of Activin/Nodal signaling is less clear. To determine the target genes of Activin/Nodal-Smad2 signaling in undifferentiated embryonic stem cells, changes in gene expression were examined following stimulation with recombinant Activin (2 hours) or after inhibition of Activin/Nodal with SB431542 (24 hours) using defined media culture conditions with LIF and 20 ng/mL BMP4. SB431542 is a specific inhibitor of ALK4/5/7 receptors and antagonizes both Activin and Nodal signaling. Via western analysis, Activin stimulation increased pSmad2 in ES cells after 2 hours, and treatment with SB431542 for 24 hours virtually eliminated pSmad2. Total Smad2 expression remained unchanged through these manipulations. RNA from cells treated with Activin or SB431542 was extracted by standard methods with Qiagen RNeasy columns. The RNA was analyzed with the Mouse Genome 430A Array from Affymetrix. Samples were performed in duplicate, and RNA from cells treated with Activin or SB431542 was compared to untreated embryonic stem cells.

2009-08-31 | GSE17879 | GEO

Transcription profiling of mouse embryonic stell cells to investigate Activin/Nodal signaling

Project description:Members of the transforming growth factor (TGF)-Î² superfamily play essential roles in the pluripotency, self-renewal, and differentiation of embryonic stem cells. While bone morphogenic proteins maintain pluripotency of undifferentiated mouse ES cells, the role of Activin/Nodal signaling is less clear. To determine the target genes of Activin/Nodal-Smad2 signaling in undifferentiated embryonic stem cells, changes in gene expression were examined following stimulation with recombinant Activin (2 hours) or after inhibition of Activin/Nodal with SB431542 (24 hours) using defined media culture conditions with LIF and 20 ng/mL BMP4. SB431542 is a specific inhibitor of ALK4/5/7 receptors and antagonizes both Activin and Nodal signaling. Via western analysis, Activin stimulation increased pSmad2 in ES cells after 2 hours, and treatment with SB431542 for 24 hours virtually eliminated pSmad2. Total Smad2 expression remained unchanged through these manipulations. RNA from cells treated with Activin or SB431542 was extracted by standard methods with Qiagen RNeasy columns. The RNA was analyzed with the Mouse Genome 430A Array from Affymetrix. Samples were performed in duplicate, and RNA from cells treated with Activin or SB431542 was compared to untreated embryonic stem cells. Experiment Overall Design: RNA from cells treated with Activin or SB431542 was extracted by standard methods with Qiagen RNeasy columns. The RNA was analyzed with the Mouse Genome 430A Array from Affymetrix. Samples were performed in duplicate, and RNA from cells treated with Activin or SB431542 was compared to untreated embryonic stem cells.

2009-09-12 | E-GEOD-17879 | biostudies-arrayexpress

Mus musculus

Project description:Influence of SB431542 on J1 mouse embryonic stem cells growth

| PRJNA240819 | ENA

Mus musculus

Project description:Activin/Nodal signaling in mouse embryonic stem cells

| PRJNA119997 | ENA

SNON and Arkadia (RNF111) regulate TGFβ-SMAD2/3 dose-dependent responses

Project description:The purpose of this RNA-seq experiment was to identify Activin A target genes that were differentially expressed in wild type and Arkadia null mouse embryonic stem cells (ESC). We performed the RNA sequencing experiment in two different wt and Arkadia-/- ESC under conditions of signalling inhibition SB431542 (SB; T0) and signalling stimulation with Activin A for 3 and 6 hours (T3 and T6 respectively).

2018-08-02 | GSE118005 | GEO

Mus musculus

Project description:BMP and Activin treatment of mouse extraembryonic endoderm (XEN) cells

| PRJNA147383 | ENA

Mus musculus embryonic germ cell transcriptome

Project description:Identification of the mouse embryonic germ cell transcriptome

2015-08-13 | GSE70360 | GEO

Transcription profiling of human embryonic stems cells grown in chemically defined medium supplemented with Activen and FGF or FGF2 and SB431542 to identify genes controlled by Activin/Nodal signalling

Project description:Human Embryonic Stem Cells (hESC) grown in chemically defined medium (CDM) supplemented with Activin and FGF were compared to hESCs grown for 48 hours in CDM supplemented with FGF2 and SB431542, an inhibitor of Activin/Nodal signalling. The objective of this experiment was to discover the identity of genes controlled by Activin/Nodal signalling in hESCs.<br><br>

2009-03-14 | E-MEXP-1741 | biostudies-arrayexpress

Mus musculus Dazl-deficient fetal ovary transcriptome

Project description:Identification of the mouse embryonic germ cell Dazl-/- transcriptome

2015-08-13 | GSE70358 | GEO