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Mixed sample

ABSTRACT: Mixture of 8 species: Escherichia coli MG1655, Bacteroides caccae ATCC 43185, Bacteroides ovatus ATCC 8483, Bacteroides thetaiotaomicron VPI-5482, Bacteroides vulgatus ATCC 8492, Collinsella aerofaciens ATCC 25986, Clostridium bolteae ATCC BAA-613, a

PROVIDER: PRJNA343143 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
		Other
	SRR4429750_subreads.fastq.gz	Fastqsanger.gz
	SRR4429751_subreads.fastq.gz	Fastqsanger.gz
	SRR4429752_subreads.fastq.gz	Fastqsanger.gz
	SRR4429755_subreads.fastq.gz	Fastqsanger.gz

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Project description:Analysis of six types of single bacterial species, including Bacteroides vulgatus ATCC 8482,Bacteroides ovatus ATCC 8483, Bacteroides uniformis ATCC 8492, Blautia hydrogenotrophica DSM 10507, Bacteroides thetaiotaomicron ATCC 29148, Escherichia coli DSM 101114

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Project description:We used Affymetrix GeneChips to determine the physiological differences between biofilm and planktonic cells of Bacteroides thetaiotaomicron strain VPI-5482 (ATCC 29148) by comparing gene expression. For this purpose, B. thetaiotaomicron cells were grown in sterile, continuous flow bioreactors fed with tryptone, yeast extract, glucose (TYG) medium. The bioreactors were controlled at a temperature of 37C using a water jacket and a recirculating water heater. After 8 hours post-inoculation, planktonic cells were harvested from the bulk solution in the bioreactor, and after 8 days post-inoculation, the biofilm was scraped from the carbon paper. RNA was harvested from both biofilm and planktonic populations. RNA was extracted by a phenol:chloroform method and purified with a Qiagen RNA Easy mini-kit. Overall growth conditions are summarized above. The experimental conditions were: biofilm (BF), and planktonic (PL).

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Bacteroides thetaiotaomicron VPI-5482

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