Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Human naive pluripotent stem cells exhibit X chromosome dampening and X-inactivation

ABSTRACT: Human naive pluripotent stem cells exhibit X chromosome dampening and X-inactivation

PROVIDER: PRJNA343940 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR4289950.fastq.gz	Fastqsanger.gz
	SRR4289960.fastq.gz	Fastqsanger.gz
	SRR4289970.fastq.gz	Fastqsanger.gz
	SRR4289951.fastq.gz	Fastqsanger.gz
	SRR4289961.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 597

Similar Datasets

Human naïve pluripotent stem cells exhibit X chromosome dampening and X-inactivation (RRBS)

Project description:Naïve human embryonic stem cells (hESCs) can be derived from primed hESCs or directly from blastocysts, but their X-chromosome state has remained unresolved. We found that the inactive X-chromosome (Xi) of primed hESCs was reactivated in naïve culture conditions. Similar to cells of the blastocyst, resulting naive cells exhibited two active X-chromosomes with XIST expression and chromosome-wide transcriptional dampening, and initiated XIST-mediated X-inactivation upon differentiation. Both establishment and exit from the naïve state (differentiation) happened via an XIST-negative XaXa intermediate. Together, these findings identify a cell culture system for functionally exploring the two X-chromosome dosage compensation processes in early human development: X-dampening and X-inactivation. Furthermore, the naïve state reset Xi abnormalities of primed hESCs, providing cells better suited for downstream applications. However, naïve hESCs displayed differences to the embryo because XIST expression was predominantly mono-allelic instead of bi-allelic, and X-inactivation was non-random, indicating the need for further culture improvement.

2016-10-21 | GSE87235 | GEO

Human naïve pluripotent stem cells exhibit X chromosome dampening and X-inactivation (RNA-Seq)

2016-10-21 | GSE87231 | GEO

Human naïve pluripotent stem cells exhibit X chromosome dampening and X-inactivation (single cell RNA-Seq)

2016-10-21 | GSE87237 | GEO

Xist-mediated Chromosome Inactivation

Project description:Xist-mediated Chromosome Inactivation

| PRJNA489702 | ENA

Spen regulates X chromosome inactivation

Project description:Spen regulates X chromosome inactivation

| PRJNA544935 | ENA

Human naive pluripotent stem cells exhibit X chromosome dampening and X-inactivation

Project description:This SuperSeries is composed of the SubSeries listed below.

2016-10-21 | GSE87239 | GEO

Project description:Human naïve pluripotent stem cells exhibit X chromosome dampening and X-inactivation

| PRJNA343135 | ENA

Molecular Criteria for Defining the Naive Human Pluripotent State

Project description:Naive human pluripotent cells have been isolated in culture, but it remains unclear to what extent they resemble pluripotent cells in vivo. Here we present three lines of evidence indicating that naive pluripotent stem cells generated by small molecules share defining molecular signatures with the human preimplantation embryo. First, a comprehensive analysis of the transposcriptome reveals that naive human cells display a unique transposon expression profile of cleavage stage embryos. Second, induction of naive pluripotency is accompanied by a genome-wide depletion in DNA methylation that is reversible upon differentiation except at imprinted regions. Third, allele-specific analysis demonstrates that female naive cells exhibit an X chromosome signature of the human preimplantation embryo. However, when introduced into mouse embryos the naive cells failed to efficiently generate interspecies chimeras. Our work provides a set of molecular benchmarks for evaluating the developmental identity of distinct human pluripotent states captured in vitro.

2016-07-18 | GSE75868 | GEO

Spen regulates X chromosome inactivation (CUT&RUN)

Project description:Spen regulates X chromosome inactivation (CUT&RUN)

| PRJNA544936 | ENA

Homo sapiens

Project description:Human naïve pluripotent stem cells exhibit X chromosome dampening and X-inactivation (RRBS)

| PRJNA343937 | ENA