Project description:Mesenchymal stem/stromal cells (MSCs) were harvested from subcutaneous adipose tissue of patients with obesity or healthy controls and expanded for 3-4 passages, and 5hmC profiles were examined through hydroxymethylated DNA immunoprecipitation sequencing (hMeDIP-seq). We hypothesized that obesity and cardiovascular risk factors induce functionally-relevant, locus-specific changes in overall exonic coverage of 5hmC in human adipose-derived MSCs.
Project description:The circadian system regulates many biological processes, including the transcriptome. An issue with human research is that serial sampling of tissues is difficult, and thus a temporal profile of the transcriptome is hard to generate. Using a technique developed at Surrey, biopsies of subcutaneous adipose tissue could be taken over multiple time points in one individual under highly controlled laboratory conditions. This study aimed to discover if the transcriptome of human subcutaneous adipose tissue was under circadian regulation, and whether key metabolic processes were under circadian variation.
