Project description:Gene expression profiles of tumor epithelium and tumor-associated stroma of HER2+ breast tumors, plus normal epithelium and stroma from HER2+ and HER2- breast tumors
Project description:Histologically normal breast epithelium and stroma were laser capture microdissected from breast reduction specimens and from specimens of invasive ductal carcinoma. The objective of the study was to compare normal reduction tissues to tissues adjacent to I.D.C. to determine whether adjacent normal tissues contained expression profiles correlated with characteristics of the primary tumor and to identify markers of normal epithelium and stroma. Keywords: disease state analysis
Project description:Ductal carcinoma in situ (DCIS) is a precursor lesion that can give rise to invasive breast cancer (IBC). It has been proposed that both the nature of the lesion and the tumor microenvironment play key roles in progression to IBC. Here, laser capture microdissected tissue samples from epithelium and stroma in normal breast, pure DCIS, and pure IBC were employed to define key gene expression profiles associated with disease progression. Tumor and matching stroma were profiled for 9 DCIS patients, 10 IBC patients, and 3 normal breast. Differential gene expression was evaluated for paired normal stroma versus normal epitelium samples, paired DCIS stroma versus DCIS epitelium samples, paired IBC stroma versus IBC epitelium, IBC stroma versus DCIS stroma, and IBC epithelium versus DCIS epithelium.
Project description:Stratification of breast cancers into subtypes are generally based on immune assays on tumor cells and/or mRNA expression of tumor cell enriched tissues. Here, we have laser microdissected tumor epithelium and tumor stroma from 24 breast cancer biopsies (12 luminal-like and 12 basal-like). We hypothesized that the stromal proteome would separate patients with breast into groups independently of the traditional epithelial based subtypes.
Project description:Histologically normal breast epithelium and stroma were laser capture microdissected from breast reduction specimens and from specimens of invasive ductal carcinoma. The objective of the study was to compare normal reduction tissues to tissues adjacent to I.D.C. to determine whether adjacent normal tissues contained expression profiles correlated with characteristics of the primary tumor and to identify markers of normal epithelium and stroma. Experiment Overall Design: common reference design. 66 samples replicated twice as dye swaps generating 132 arrays.
Project description:Triple-negative primary breast cancer tumors, rich in inflammatory stroma, were laser microdissected and global mRNA expression was analyzed in stroma compartment, cancer cell compartment, and in total tumor tissue. Three FFPE breast cancer tumors wer laser microdissected and the cancer cell and stroma compartments were collected. A piece of t
Project description:Triple-negative primary breast cancer tumors, rich in inflammatory stroma, were laser microdissected and global mRNA expression was analyzed in stroma compartment, cancer cell compartment, and in total tumor tissue.
Project description:Ductal carcinoma in situ (DCIS) is a precursor lesion that can give rise to invasive breast cancer (IBC). It has been proposed that both the nature of the lesion and the tumor microenvironment play key roles in progression to IBC. Here, laser capture microdissected tissue samples from epithelium and stroma in normal breast, pure DCIS, and pure IBC were employed to define key gene expression profiles associated with disease progression.
