Project description:We profiled miRNAs in gingival crevicular fluid (GCF) by a PCR-based method that yielded quantitative measures of more than 600 miRNAs. We found that miRNA profiles in GCF of periodontitis patients are distinct from those of healthy controls.
Project description:The human gingival crevicular fluid proteome and metaproteome of periodontitis are investigated, to shed light on the factors that mediate the host-microbiota interactions in the pathogenesis of periodontitis.
Project description:Inflammatory periodontal disease (periodontitis) is widespread in dogs. This study aimed to evaluate site-specific changes in the canine gingival crevicular fluid (GCF) proteome during the longitudinal progression from very mild gingivitis to mild periodontitis. Periodontitis diagnosis in dogs requires anaesthesia, our ultimate aim was to develop a periodontitis diagnostic that could be applied to samples taken from conscious dogs. The objective of this work was to identify potential biomarkers of periodontal disease progression in the GCF of dogs.
Project description:Saliva, gingival crevicular fluid (GCF), and serum are common sources for studying host response and oral microbiota in periodontal patients. This cross-sectional study aimed to compare the proteomic signatures of periodontitis patients using full-mouth periodontal sampling with those obtained from serum and saliva samples. This study analysed proteome profiles from these biological sources in three systemically healthy, non-smoking patients with stage III, and grade C periodontitis.
Project description:Aim: To provide an exploratory proteomic characterization of periodontal disease-related immune pathways by analyzing gingival crevicular fluid, revealing subtle proteomic changes accompanying disease progression. Materials and methods: Gingival crevicular fluid samples were collected from normal (control group), gingivitis, and periodontitis cohorts. Seventy-two samples were investigated, including those from normal (n=12), gingivitis (n=30) and periodontitis (n=30) groups. Samples were analyzed using liquid chromatography–mass spectrometry and sequential statistical analyses including differential expression, weighted gene co-expression network analysis, and protein–protein interaction networks, evaluated with receiver operating characteristic curve analyses. Results: We identified 649 proteins. Differential expression analysis revealed several regulated proteins, including RAC2, S100A12, and LCN2, in the periodontitis group. Weighted gene co‐expression network analysis clustered six protein modules: M1 module was enriched in complement proteins and prominent in gingivitis, whereas neutrophil-related M2 and M5 modules were correlated with periodontitis severity. Protein–protein interaction network analyses revealed hub proteins, including RAC2 and S100A12, highlighting their functional associations. Receiver operating characteristic analysis supported their within-cohort discriminatory potential for selected proteins. Conclusion: This exploratory study suggested the pathophysiological differences in proteome between gingivitis and periodontitis, establishing a foundation for future investigations and hypothesis-driven research.
Project description:Complement inhibition has been successfully used to treat periodontitis in animal models. However, proteome studies that investigated the role of complement C3 were missing. The objective of this study was to characterize local tissue exudate (“gingival crevicular fluid”; GCF) changes after administration of a potent C3 inhibitor (Cp40, also known as AMY-101) to cynomolgus monkeys (Macaca fascicularis) with established periodontitis. Cp40 was administered locally in the maxillary gingival tissue either once per week (n=5 animals) or three times per week (n=10 animals), for six weeks followed by another six weeks of observation in the absence of treatment. The GCF were collected for liquid chromatography–mass spectrometry analysis (LC-MS/MS).
Project description:Gingival Crevicular Fluid, a plasma-derived exudate present in the gingival crevice was collected from deciduous, exfoliating and permanent teeth from 20 children (60 samples) with the aim to characterize and quantify by a mass spectrometry based top-down proteomic approach, the peptide/proteins in the fluid and verify possible variations occurring during the exfoliating process. The results obtained confirmed the presence in Gingival Crevicular Fluid of α-Defensins 1-4, Thymosin β4 and Thymosin β10, as described in previous works and revealed the presence of other interesting peptides never described before in Gingival Crevicular Fluid, such as specific fragments of α-1-antitrypsin, α-1-antichymotrypsin, Thymosin β4 and Thymosin β10 fragments, Fibrinopeptide A, Fibrinopeptide B, S100A, LVV Hemorphin-7 (hemoglobin chain β fragment), as well as some other peptides deriving from α and β subunits of hemoglobin. Statistical analysis evidenced different levels in 5 proteins/peptides in the three groups in particular with higher level in exfoliating teeth. Our study demonstrate that an in-depth analysis of a biological fluid like Gingival Crevicular Fluid, present in small amount, can provide useful information for the understanding of different biological processes like teeth eruption.
Project description:Thirteen healthy men diagnosed with periodontitis were enrolled. Progression of periodontitis was monitored by clinical attachment level (CAL) changes at the site level over twelve weeks via weekly clinical evaluations and gingival crevicular fluid (GCF) samples were collected using cellulose strips for subsequent mass spectrometry analysis. Progression group (PG) included 18 GCF samples matching sites with disease progression (CAL ≥ 2 mm), while the non-Progression group (NP) included 18 GCF samples from stable sites (No CAL changes) matched in the same patients. Clinical metrics, HPRLC-MS/MS, in silico methods, and bioinformatics tools, were employed to analyze the proteome of GCF, revealing the significant occurrence of ferroptosis during clinical progression of periodontitis.
