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Bos taurus

ABSTRACT: RNA-seq analysis of differential gene expression in rumen papillae from Holstein Friesian bulls undergoing diet restriction and compensatory growth

PROVIDER: PRJNA350513 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR4449880.fastq.gz	Fastqsanger.gz
	SRR4449890.fastq.gz	Fastqsanger.gz
	SRR4449900.fastq.gz	Fastqsanger.gz
	SRR4449910.fastq.gz	Fastqsanger.gz
	SRR4449881.fastq.gz	Fastqsanger.gz

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Project description:RNA-seq analysis of differential gene expression in muscle from Holstein Friesian bulls undergoing diet restriction and compensatory growth

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RNA-seq analysis of differential gene expression in rumen papillae from Holstein Friesian bulls undergoing diet restriction and compensatory growth

Project description:The objective of this study was to examine the effect of dietary restriction and subsequent re-alimentation induced compensatory growth on the global gene expression profile of ruminal epithelial papillae. Holstein Friesian bulls (n=38) were assigned to one of two groups: restricted feed allowance (RES; n=19) for 125 days (Period 1) followed by ad libitum access to feed for 55 days (Period 2) or (ii) ad libitum access to feed throughout (ADLIB; n=19). All bulls received the same diet of 70% concentrate 30% grass silage through out the experimental trial,with the amount of feed provided different dependent on each treatment group. At the end of Period 1, 9 animals from each treatment group were slaughtered, with 10 animals from each treatment slaughtered at the end of Period 2. Rumen epithelium was collected from all animals within thirty minutes of slaughter. RNA was extracted and rumen epithelium gene expression was examined using RNAseq technology on all samles collected (end of Period 1: 9 samples each from ADLIB and RES groups; end of Period 2: 10 samples each from ADLIB and RES groups). Genes identified as differentially expressed in response to both dietary restriction and subsequent compensatory growth included those involved in processes such as cellular interactions and transport, protein folding and gene expression, as well as immune response. This information can be exploited in genomic breeding programmes to assist selection of cattle with a greater ability to compensate following a period dietary restriction.

2017-06-09 | GSE89162 | GEO

Bos taurus

Project description:Effect of Dietary Grain on Rumen Papillae Gene Expression in Holstein Dairy Cows

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Bos taurus

Project description:Exome of Holstein Friesian Cows

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Bos taurus

Project description:Effect of early calf-hood nutrition on the transcriptomic profile of subcutaneous adipose in Holstein-Friesian bulls

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Project description:Effect of early calf-hood nutrition on the transcriptomic profile of the HPT axis in Holstein-Friesian bulls

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Project description:Semen miRNA in Holstein Friesian crossbred cattle

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Transcription profiling of Bos taurus skin of tick naive and tick-infested Holstein-Friesian and Brahman breeds

Project description:This experiment was undertaken to document changes in gene expression in the skin of tick-resistant Brahman (Bos indicus) and tick-susceptible Holstein-Friesian (Bos taurus) cattle prior to, and following, infestation with the cattle tick Rhipicephalus (Boophilus) microplus Experiment Overall Design: RNA was extracted from skin samples of tick-naÃ¯ve cattle (animals with no previous R.microplus exposure) and tick-infested cattle after a period of successive, heavy infestations with R. microplus. Skin samples taken from tick-infested animals were taken at sites where tick larvae (approximately 24 h old) were attached to the skin sample. Skin samples were of 8 mm diameter and full skin thickness (approximately 10 mm). RNA samples from 12 animals (3 tick-naive Holstein-Friesian, 3 tick-naive Brahman, 3 tick-infested Holstein-Friesian and 3 tick-infested Brahman) were processed and hybridised to individual slides.

2009-04-11 | E-GEOD-15342 | biostudies-arrayexpress

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