Project description:To understand the mechanism of isopropanol tolerance of Escherichia coli for improvement of isopropanol production, we performed genome re-sequencing and transcriptome analysis of isopropanol tolerant E. coli strains obtained from parallel adaptive laboratory evolution under IPA stress.
Project description:Several groups have shown that through evolution experiments, tolerance and resistance evolved rapidly under cyclic antibiotic treatment. In other words, intermittent antibiotic exposure performed in a typical adaptive laboratory evolution (ALE) experiments will “train” the bacteria to become tolerant/resistant to the drug. Using this experimental strategy, we performed in vitro laboratory evolution in MRSA using daptomycin, and mine novel daptomycin tolerance and resistance mutants, which were isolated at specific time points during the evolution experiments. Three daptomycin-tolerant isolates with different tolerance level were generated from our laboratory evolution (TOL2 and TOL5 with a mild-tolerance phenotype, and TOL6 with a high-tolerance phenotype). They all bear mutations at different genes, and have no increase in MIC towards daptomycin. Besides, we also isolated three daptomycin-resistant isolates (RES1, RES2, RES3) that have a single point mutation in the same gene, mprF, but at different locations, leading to an increased MIC towards daptomycin. Through proteomics, we uncovered the differential adaptation strategies of these daptomycin tolerant and resistant MRSA strains, and how they respond differently to antibiotics compared to the ancestral wild-type.
2022-07-14 | PXD026741 | Pride
Project description:Adaptive Laboratory Evolution of Escherichia coli PB11
| PRJNA593332 | ENA
Project description:Adaptive Laboratory Evolution of Escherichia coli PB11
| PRJNA593336 | ENA
Project description:Adaptive Laboratory Evolution of Escherichia coli PB11
| PRJNA593068 | ENA
Project description:Adaptive Laboratory Evolution of Escherichia coli PB11
| PRJNA593257 | ENA
Project description:Adaptive Laboratory Evolution of Escherichia coli PB11
| PRJNA593331 | ENA
Project description:Adaptive Laboratory Evolution of Escherichia coli PB11
| PRJNA593345 | ENA
Project description:Elucidating the mechanisms of styrene tolerance in Gluconobacter oxydans using adaptive laboratory evolution
