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Kineococcus radiotolerans

ABSTRACT: Radiation-resistant bacterium

PROVIDER: PRJNA35303 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	SRR10145199_1.fastq.gz	Fastqsanger.gz
	SRR10145199_2.fastq.gz	Fastqsanger.gz
	SRR1137094_1.fastq.gz	Fastqsanger.gz
	SRR1137094_2.fastq.gz	Fastqsanger.gz

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Kineococcus radiotolerans

Project description:RNA sequencing of Kineococcus radiotolerans transcriptome in response to ionizing radiation

| PRJNA221158 | ENA

RNA sequencing of Kineococcus radiotolerans transcriptome in response to ionizing radiation

2013-09-24 | GSE51096 | GEO

RNA sequencing of Kineococcus radiotolerans transcriptome in response to ionizing radiation

Project description:In this study, approximately 36 and 29 million raw reads of two samples, namely radiation treated strain and its untreated control, are acquired from the sequencing platform. And 143 genes are screened out with the differential expression (DE) analysis. Investigation of the differential expression between the radiation reduced sample and the wild-type sample in control.

2013-09-24 | E-GEOD-51096 | biostudies-arrayexpress

Proteomic and physiological responses of Kineococcus radiotolerans to copper.

Project description:Copper is a highly reactive, toxic metal; consequently, transport of this metal within the cell is tightly regulated. Intriguingly, the actinobacterium Kineococcus radiotolerans has been shown to not only accumulate soluble copper to high levels within the cytoplasm, but the phenotype also correlated with enhanced cell growth during chronic exposure to ionizing radiation. This study offers a first glimpse into the physiological and proteomic responses of K. radiotolerans to copper at increasing concentration and distinct growth phases. Aerobic growth rates and biomass yields were similar over a range of Cu(II) concentrations (0-1.5 mM) in complex medium. Copper uptake coincided with active cell growth and intracellular accumulation was positively correlated with Cu(II) concentration in the growth medium (R(2)=0.7). Approximately 40% of protein coding ORFs on the K. radiotolerans genome were differentially expressed in response to the copper treatments imposed. Copper accumulation coincided with increased abundance of proteins involved in oxidative stress and defense, DNA stabilization and repair, and protein turnover. Interestingly, the specific activity of superoxide dismutase was repressed by low to moderate concentrations of copper during exponential growth, and activity was unresponsive to perturbation with paraquot. The biochemical response pathways invoked by sub-lethal copper concentrations are exceptionally complex; though integral cellular functions are preserved, in part, through the coordination of defense enzymes, chaperones, antioxidants and protective osmolytes that likely help maintain cellular redox. This study extends our understanding of the ecology and physiology of this unique actinobacterium that could potentially inspire new biotechnologies in metal recovery and sequestration, and environmental restoration.

| S-EPMC2928746 | biostudies-literature

Kineococcus radiotolerans

Project description:Kineococcus radiotolerans Genome sequencing and assembly

| PRJNA288191 | ENA

Kineococcus radiotolerans SRS30216 = ATCC BAA-149

Project description:Radiotolerant bacterium isolated from a radioactive waste basin.

| PRJNA10689 | ENA

Rubrobacter radiotolerans DSM 5868

Project description:High radiation-resistant, pink-pigmented, thermophilic bacteria

| PRJNA37865 | ENA

Survival in nuclear waste, extreme resistance, and potential applications gleaned from the genome sequence of Kineococcus radiotolerans SRS30216.

Project description:Kineococcus radiotolerans SRS30216 was isolated from a high-level radioactive environment at the Savannah River Site (SRS) and exhibits gamma-radiation resistance approaching that of Deinococcus radiodurans. The genome was sequenced by the U.S. Department of Energy's Joint Genome Institute which suggested the existence of three replicons, a 4.76 Mb linear chromosome, a 0.18 Mb linear plasmid, and a 12.92 Kb circular plasmid. Southern hybridization confirmed that the chromosome is linear. The K. radiotolerans genome sequence was examined to learn about the physiology of the organism with regard to ionizing radiation resistance, the potential for bioremediation of nuclear waste, and the dimorphic life cycle. K. radiotolerans may have a unique genetic toolbox for radiation protection as it lacks many of the genes known to confer radiation resistance in D. radiodurans. Additionally, genes involved in the detoxification of reactive oxygen species and the excision repair pathway are overrepresented. K. radiotolerans appears to lack degradation pathways for pervasive soil and groundwater pollutants. However, it can respire on two organic acids found in SRS high-level nuclear waste, formate and oxalate, which promote the survival of cells during prolonged periods of starvation. The dimorphic life cycle involves the production of motile zoospores. The flagellar biosynthesis genes are located on a motility island, though its regulation could not be fully discerned. These results highlight the remarkable ability of K radiotolerans to withstand environmental extremes and suggest that in situ bioremediation of organic complexants from high level radioactive waste may be feasible.

| S-EPMC2587704 | biostudies-literature

Kineococcus radiotolerans strain:AS2.23

Project description:Kineococcus radiotolerans AS2.23 genome sequencing

| PRJNA546676 | ENA

Deinococcus radiodurans

Project description:Radiation-resistant bacterium

| PRJNA35079 | ENA