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Comparison of bovine embryo transcriptome at 16-cell (96 hpi) and blastocysts (192 hpi) stages in presence of co-culture systems (BOEC or VERO) in SOF medium

ABSTRACT: Comparison of bovine embryo transcriptome at 16-cell (96 hpi) and blastocysts (192 hpi) stages in presence of co-culture systems (BOEC or VERO) in SOF medium

PROVIDER: PRJNA354781 | ENA |

REPOSITORIES: ENA

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Comparison of bovine blastocysts (192 hpi) embryo transcriptome in presence of co-culture systems (BOEC or VERO) in SOF medium

Project description:To analyze impact of co-culture presence and type of feeder cell lines, we compared the transcriptome of bovine blastocyst (192 hpi) using a new custom bovine microarray.

2016-12-15 | GSE90451 | GEO

Comparison of bovine embryo transcriptome at 16-cell (96 hpi) and blastocysts (192 hpi) stages in presence of co-culture systems (BOEC or VERO) in SOF medium

Project description:This SuperSeries is composed of the SubSeries listed below.

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Project description:Comparison of bovine blastocysts (192 hpi) embryo transcriptome in presence of co-culture systems (BOEC or VERO) in SOF medium

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Comparison of bovine 16-cell (96 hpi) embryo transcriptome in presence of co-culture systems (BOEC or VERO) in SOF medium

Project description:To analyze impact of co-culture presence and type of feeder cell lines, we compared the transcriptome of bovine 16-cell (96 hpi) using a new custom bovine microarray.

2016-12-15 | GSE90114 | GEO

Transcriptome Analysis Of Bovine Expanded Blastocysts Produced In Vivo Vs In Vitro Sof Opu

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The impact of exposure to serum lipids during in vitro culture on the transcriptome of bovine blastocysts

2014-01-09 | GSE53905 | GEO

Transcriptome Analysis Of Bovine Expanded Blastocysts Produced In Vivo Vs In Vitro Sof Opu

Project description:Comparison of gene expression from expanded bovine blastocysts collected 7 days after fertilization and produced in vivo vs in vitro-SOF-OPU Two kinds of 7 days post fertilization bovine embryos, in-vivo 7 days blastocysts vs. In vitro 7 days blastocysts, Biological replicates: 4 in-vitro, 4 in-vivo, protocol,extract and semen shared. Dye swap.

2012-03-31 | E-GEOD-35025 | biostudies-arrayexpress

Deletion of HCMV US17 alters host immune genes at 12 hpi and ER stress genes at 96 hpi.

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2013-11-30 | GSE50955 | GEO

The impact of exposure to serum lipids during in vitro culture on the transcriptome of bovine blastocysts

Project description:EtOH-Lipid form serum have been added in BSA-supplemented SOF during post-compaction development of IVP bovine embryos. Lipid-treated blastocysts were compared with control treated blastocysts (no lipid addition to BSA, EtOH balanced). Amplified anti-sense RNA from each sample (4 pools of control and 4 pools of treated blastocysts) were labeled then hybridized on oligonucleotide microarrays following a dye-swap design (ex: pool 1 ctl in red vs. pool 1 treatment in green and then pool 1 ctl in green vs. pool 1 treatment in red).

2014-01-09 | E-GEOD-53905 | biostudies-arrayexpress

Expression data from Arabidopsis wild-type, sicy-192, and sicy-192 gun1-101 plants

Project description:Plastid gene expression (PGE) acts as a signal that regulates the expression of photosynthesis-associated nuclear genes (PhANGs) via GENOMES UNCOUPLED1 (GUN1)-dependent retrograde signaling. We recently isolated Arabidopsis sugar-inducible cotyledon yellow-192 (sicy-192), a gain-of-function mutant of plastidic invertase (INV-E), and showed that following the treatment of this mutant with sucrose, the expression of PhANGs decreased while that of nitrate reductase 1 (NIA1) increased. Plastid-encoded RNA polymerase (PEP)-dependent PGE was markedly suppressed in the sicy-192 mutant by the sucrose treatment. A double mutant of sicy-192 and gun1-101, a null mutant of GUN1, revealed that metabolic perturbation in the sucrose-treated sicy-192 mutant was, at least in part, dependent on GUN1. To confirm whether there is a relationship between plastid sugar metabolism and nuclear gene expression, we performed a microarray analysis using Suc-treated 3 genotypes consisting of wild-type, sicy-192, and sicy-192 gun1-101 plants.

2013-09-14 | GSE50857 | GEO

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