ABSTRACT: Comparative expression analysis of the Arabidopsis karrikin (KAR)-receptor mutant, kai2-2, and wild type (WT) plants (Col-0) under dehydration conditions
Project description:To understand the role of KAR-signaling components in water stress response, we have carried out comparative expression analysis of the KAR-receptor kai2-2 mutant and WT plants under dehydration and well-watered (control) conditions. Aligent’s whole Arabidopsis Gene Expression Microarray (G2519F-021169, V4, 4x44K) was used.
Project description:In this study, we investigated the potential role of the karrikin receptor KARRIKIN INSENSITIVE2 (KAI2) in the response of Arabidopsis seedlings to high temperature stress.
Project description:Comparative expression analysis of the Arabidopsis strigolactone (SL)-receptor mutant, d14-1, and wild type (WT) plants (Col-0) under dehydration conditions.
Project description:To understand the role of SL-signaling components in water stress response, we have carried out comparative expression analysis of the SL-receptor d14-1 mutant and WT plants under dehydration and well-watered (control) conditions. Aligent’s whole Arabidopsis Gene Expression Microarray (G2519F-021169, V4, 4x44K) was used.
Project description:Objective: to compare and contrast the effects of exogenous application of karrikins KAR1 and KAR2, and the strigolatone analogue rac-GR24, on gene expression in shoot tissue of seedlings of Brachpodium distachyon (false brome). The experiment compared the effects on three genotypes: wild type Bd21-3, and two mutant alleles for the Brachypodium orthoologue of the karrikin receptor KARRIKIN INSENSITIVE2 (KAI2; Bradi1g15880.1). These alleles were designated Bdkai2-1 and Bdkai2-2.
Project description:To understand the role of the Arabidopsis type-B Response Regulators ARR1, ARR10 and ARR12 in water stress response, we have carried out comparative expression analysis of the arr1,10,12 mutant and WT plants under dehydration and well-watered (control) conditions. Agilentâs whole Arabidopsis Gene Expression Microarray (G2519F-021169, V4, 4x44K) was used. Two-week-old WT and arr1,10,12 mutant plants were transferred from GM plates to soil and grown for 10 additional day. Aerial portions of 24-d-old plants were detached and exposed to dehydration on KimTowel papers for 0 (well-watered, control), 2 and 4 h. Rosette leaves collected in 3 biological repeats from arr1,10,12 and WT plants treated by dehydration for 0, 2 and 4 h were used for microarray and expression analyses. Total RNA was prepared and used for the microarray hybridization. Three independent biological replicates were used for each plant sample.
Project description:Comparative transcriptomic analysis of Arabidopsis thaliana yda11 plants (in Col-0 background), and wild-type plants (Col-0) non-infected or infected with the necrotrophic fungal pathogen Plectosphaerella cucumerina BMM (PcBMM)
Project description:In terrestrial ecosystems plants take up phosphate predominantly via association with arbuscular mycorrhizal fungi (AMF). We discovered that the loss of responsiveness to AMF in the rice deletion mutant hebiba is encoded by the alpha/beta fold hydrolase, DWARF 14 LIKE (D14L), which is one of the 26 deleted genes. It is a component of an intracellular receptor complex involved in the detection of the smoke-compound karrikin. On the basis of the early and pronounced hebiba mutant phenotype, we hypothesized that functional D14L is required for the perception of AM fungi prior to contact. Germinated spore exudates of AMF activate pre-contact plant responses. Therefore, we used RNAseq to monitor the transcriptional changes of hebiba and wild type roots in response to germinated spore exudates, and also karrikin, over the first 24 hours post treatment. WT seedlings were treated with GSE, Karrikin or a mock and iho seedlings with GSE or a mock. Root material was collected for sequencing at 0, 3, 6, 9, 12 and 24 hours. This gave a total of 27 samples (WT+Mock: 6, WT+GSE: 5, WT+Karrikin:5, iho+Mock:6, iho+GSE: 5).
Project description:Comparative expression analysis of the Arabidopsis CK-signaling ahp4-1 mutant and wild type (WT) plants (Col-0) under dehydration conditions
Project description:Plants are targets of volatile organic compounds (VOCs) released as a part of plant-plant communication, within-plant self-signaling and plant-microbe interactions. Therefore, understanding VOC perception and downstream signaling is vital for unraveling the mechanisms behind information exchange in plants, which remain largely unexplored. Using the hormone-like function of volatile terpenoids in reproductive organ development as a system with a visual marker for communication, we demonstrated that among the four petunia karrikin-insensitive receptors, PhKAI2ia stereo-specifically perceives the (-)-germacrene D signal, triggering a karrikin-like signaling cascade. This study provides new insights into plant olfaction, uncovers the role(s) of the unique intermediate clade of KAI2 receptors, illuminates the involvement of KAI2ia-dependent signaling pathway in volatile communication and gives insight into the long-standing question about the nature of potential endogenous karrikin-like ligand(s).
