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Comparative expression analysis of the Arabidopsis strigolactone (SL)-receptor mutant, d14-1, and wild type (WT) plants (Col-0) under dehydration conditions.

ABSTRACT: Comparative expression analysis of the Arabidopsis strigolactone (SL)-receptor mutant, d14-1, and wild type (WT) plants (Col-0) under dehydration conditions.

PROVIDER: PRJNA527720 | ENA |

REPOSITORIES: ENA

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Comparative expression analysis of the Arabidopsis strigolactone (SL)-receptor mutant, d14-1, and wild type (WT) plants (Col-0) under dehydration conditions.

Project description:To understand the role of SL-signaling components in water stress response, we have carried out comparative expression analysis of the SL-receptor d14-1 mutant and WT plants under dehydration and well-watered (control) conditions. Aligent’s whole Arabidopsis Gene Expression Microarray (G2519F-021169, V4, 4x44K) was used.

2020-01-18 | GSE128424 | GEO

Comparative expression analysis of the Arabidopsis strigolactone (SL)-response mutant, max2-3, and wild type (WT) plants (Col-0) under dehydration conditions.

2013-11-26 | GSE48949 | GEO

Strigolactone and cytokinin act antagonistically in regulating rice mesocotyl elongation in darkness

Project description:Strigolactones have been defined as a new group of phytohormones that regulate shoot branching. Tthe phenotypes of strigolacton-related rice (Oryza sativa) dwarf (d) mutants demonstrated that SLs inhibit mesocotyl elongation by controlling cell division. Moreover, the trans-zeatin, one of active cytokinins, content of mesocotyls was increased in the SL-deficient d10-1 and SL-insensitive d14-1 mutants. To examine if there are genes related to cytokinin-biosynthesis or -degradation among the strigolactone-responsive genes expressed in the mesocotyl, we carried out microarray analyses using d10-1, d14-1 and the wild-type grown under dark conditions, with or without pretreatment of a synthetic strigolactone analog, GR24.

2013-12-31 | GSE36271 | GEO

Comparative expression analysis of the Arabidopsis strigolactone (SL)-response mutant, max2-3, and wild type (WT) plants (Col-0) under dehydration conditions.

Project description:To understand the role of SL-signaling components in water stress response, we have carried out comparative expression analysis of the SL-response max2-3 mutant and WT plants under dehydration and well-watered (control) conditions. AligentM-bM-^@M-^Ys whole Arabidopsis Gene Expression Microarray (G2519F-021169, V4, 4x44K) was used. Two-week-old WT and max2-3 mutant plants were transferred from GM plates to soil and grown for 10 additional day. The aerial parts of 24-d-old plants were detached and exposed to dehydration on KimTowel papers for 0 (well-watered, control), 2 and 4 h. All rosette leaves of independent 24-d-old plants were collected. Total RNA was prepared and used for the microarray hybridization. Three independent biological replicates were used for each plant sample.

2013-11-26 | E-GEOD-48949 | biostudies-arrayexpress

Strigolactone and cytokinin act antagonistically in regulating rice mesocotyl elongation in darkness

Project description:Strigolactones have been defined as a new group of phytohormones that regulate shoot branching. The phenotypes of strigolacton-related rice (Oryza sativa) dwarf (d) mutants demonstrated that SLs inhibit mesocotyl elongation by controlling cell division. Moreover, the trans-zeatin, one of active cytokinins, content of mesocotyls was increased in the SL-deficient d10-1 and SL-insensitive d14-1 mutants. To examine if there are genes related to cytokinin-biosynthesis or -degradation among the strigolactone-responsive genes expressed in the mesocotyl, we carried out microarray analyses using d10-1, d14-1 and the wild-type grown under dark conditions, with or without pretreatment of a synthetic strigolactone analog, GR24. Gene expression profiles in 4-day-old mesocotyls of dwarf (d) mutants (d10-1 and d14-1) and wild-type germinated under dark conditions with or without treatment of 1 μM GR24 were analyzed. Three biological replicates were prepared for each conditions, and a total of eighteen samples were analyzed.

2013-12-31 | E-GEOD-36271 | biostudies-arrayexpress

Gene expression profiling in response to strigolactone signalling revealed by dexamethasone treatment to SL-insensitive version of SMXL7 tagged with glucocorticoid receptor (SMXL7d53-GR) in Arabidopsis hypocotyl.

Project description:Strigolactone (SL) signalling plays many roles in plants, however, the downstream responsive genes remain unclear. SUPPRESSOR OF MORE AXILLARY GROWTH2-LIKE 7 (SMXL7) is degraded upon SL signalling. Here, we generated Arabidopsis carrying a transgene SMXL7pro:SMXL7d53-GR, where a SL-insensitive version of SMXL7 is tagged with the ligand binding domain of the glucocorticoid receptor (GR). SMXL7d53-GR will be translocated to the nucleus upon dexamethasone (dex), and we profiled the gene expression in mock or dex treated plants and revealed the downstream responsive genes of SL signalling.

2024-07-01 | GSE270808 | GEO

RNAseq analysis of hvd14 SL mutant and its WT under control conditions

Project description:Although previous research has established links between strigolactone (SL) and drought, in this study, we used the barley (Hordeum vulgare) SL-insensitive mutant hvd14 (dwarf14) to scrutinize the SL-dependent mechanisms associated with water deficit response. We have employed a comprehensive approach integrating transcriptome, proteome, phytohormone analyses, and physiological data to unravel differences between wild-type and hvd14 plants when responding to drought. Here we deposited control samples, tissue collected at 25DAS (Days after sowing)

2023-06-19 | E-MTAB-12804 | biostudies-arrayexpress

RNAseq analysis of barley SL mutant hvd14 and WT under drought stress

Project description:Drought is a harsh abiotic stress, with plants possessing diverse strategies to survive periods of limited water resources. Although previous research has established links between strigolactone (SL) and drought, in this study, we used the barley (Hordeum vulgare) SL-insensitive mutant hvd14 (dwarf14) to scrutinize the SL-dependent mechanisms associated with water deficit response. We have employed a comprehensive approach integrating transcriptome, proteome, phytohormone analyses, and physiological data to unravel differences between wild-type and hvd14 plants when responding to drought.

2023-06-19 | E-MTAB-12796 | biostudies-arrayexpress

Transcriptome analysis reveals that auxin promotes strigolactone-induced adventitious root growth in the hypocotyl of melon seedlings

Project description:Strigolactone (SL) and auxin are two important phytohormones involved in plant root development, but whether they show synergistic or mutual promotion effects during adventitious root (AR) formation has not been adequately explored.

2023-07-05 | GSE234393 | GEO

Interactions of strigolactone and karrikin signaling pathways on the levels of other phytohormones in rosette leaves of Arabidopsis

Project description:To understand the combined role of SL and KAR signaling pathways in regulating levels and metabolism of other plant hormones, we performed the compare transcriptome profiling d14 kai2 and WT plants. Aligent´s whole Arabidopsis Gene Expression Microarray (G2519F-021169, V4, 4x44K) was used.

2024-04-21 | GSE264168 | GEO

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