Project description:We investigated the functional gene expression changes associated with temperature stress in two psychrophilic sea ice bacteria, Polaribacter sp. ALD9 and Shewanella sp. ALD11.

2017-03-05 | E-MTAB-5507 | biostudies-arrayexpress

Cold adaptation of tRNA nucleotidyltransferases: a tradeoff in activity, stability and fidelity

Project description:CCA-adding enzymes are highly specific RNA polymerases that synthesize and maintain the sequence CCA at the tRNA 3‘-end. Here, we investigated the impact of cold adaptation on the reactivity and specificity of CCA-adding enzymes from psychrophilic bacteria. A comparative study of the corresponding enzymes from closely related psychro-, meso-, and thermophilic Bacillales indicates that the cold-adapted enzymes show a considerable error rate during CCA synthesis, resulting in additional incorporations of C and A residues. It seems that the activity of psychrophilic CCA-adding enzymes is not only achieved at the expense of structural stability, reaction velocity and substrate affinity, but also results in a reduced polymerization fidelity.

2017-11-03 | GSE98874 | GEO

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