Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Caenorhabditis elegans

ABSTRACT: Replication maps of developing C. elegans embryos.

PROVIDER: PRJNA356401 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR5080010.fastq.gz	Fastqsanger.gz
	SRR5080011.fastq.gz	Fastqsanger.gz
	SRR5080012.fastq.gz	Fastqsanger.gz
	SRR5080013.fastq.gz	Fastqsanger.gz
	SRR5080008.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 6

Similar Datasets

The embryonic muscle transcriptome of Caenorhabditis elegans

Project description:Background: The force generating mechanism of muscle is evolutionarily ancient; the fundamental structural and functional components of the sarcomere are common to motile animals throughout phylogeny. Recent evidence suggests that the transcription factors that regulate muscle development are also conserved. Thus, a comprehensive description of muscle gene expression in a simple model organism should define a basic muscle transcriptome that is also expressed in animals with more complex body plans. To this end, we have applied Micro-Array Profiling of Caenorhabditis elegans Cells (MAPCeL) to muscle cell populations extracted from developing Caenorhabditis elegans embryos. Results: Fluorescence Activated Cell Sorting (FACS) was used to isolate myo-3::GFP-positive muscle cells, and their cultured derivatives, from dissociated early Caenorhabditis elegans embryos. Microarray analysis identified 6,693 expressed genes, 1,305 of which are enriched in the myo-3::GFP positive cell population relative to the average embryonic cell. The muscle-enriched gene set was validated by comparisons to known muscle markers, independently derived expression data, and GFP reporters in transgenic strains. These results confirm the utility of MAPCeL for cell type-specific expression profiling and reveal that 60% of these transcripts have human homologs. Conclusions: This study provides a comprehensive description of gene expression in developing Caenorhabditis elegans embryonic muscle cells. The finding that over half of these muscle-enriched transcripts encode proteins with human homologs suggests that mutant analysis of these genes in Caenorhabditis elegans could reveal evolutionarily conserved models of muscle gene function with ready application to human muscle pathologies. Keywords: embryonic muscle, myo-3::GFP

2007-07-13 | GSE8462 | GEO

The gastrula transition reorganizes replication origin selection in Caenorhabditis elegans

Project description:Some features underlying replication origin activation in metazoan cells have been identified, but little is known about their regulation during metazoan development. Using the nascent strand purification method, we identified replication origins throughout Caenorhabditis elegans embryonic development and found that the origin repertoire is thoroughly reorganized after gastrulation onset. During the pluripotent embryonic stages (pre-gastrula), potential cruciform structures and open chromatin are determinant factors to establish replication origins. The enrichment of replication origins in transcription factor binding sites and their presence inside promoters of highly transcribed genes, particularly operons, argue that transcriptional activity contributes to replication initiation before gastrulation. After the gastrula transition, when differentiation programs are set in the embryos, origins are particularly selected at enhancers, in the vicinity of CGI-like sequences, and non-coding genes. Our findings suggest that origin selection coordinates replication initiation with transcriptional programs during metazoan development.

2017-01-22 | GSE86651 | GEO

Genome-wide CPD repair maps of Caenorhabditis elegans

Project description:We have adapted the eXcision Repair-sequencing (XR-seq) method to generate single-nucleotide resolution dynamic repair maps of UV-induced cyclobutane pyrimidine dimers (CPD) photoproducts in the Caenorhabditis elegans (C. elegans) genome.

2024-04-02 | GSE262486 | GEO

Caenorhabditis elegans

Project description:C. elegans embryos: control vs. mes-2 mutant

| PRJNA112021 | ENA

Caenorhabditis elegans

Project description:Illumina sequencing of small RNAs from C. elegans embryos

| PRJNA119753 | ENA

Caenorhabditis elegans

Project description:Caenorhabditis elegans Raw sequence reads JMJD-1.2 mutant embryos

| PRJNA354814 | ENA

Caenorhabditis elegans

Project description:C. elegans embryonic timecourse in wt and mutant embryos