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ABSTRACT: Epigenomic landscapes of hESC-derived neural rosettes

PROVIDER: PRJNA356642 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5085880.fastq.gz	Fastqsanger.gz
	SRR5085881.fastq.gz	Fastqsanger.gz
	SRR5085872.fastq.gz	Fastqsanger.gz
	SRR5085882_1.fastq.gz	Fastqsanger.gz
	SRR5085882_2.fastq.gz	Fastqsanger.gz

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Transcription profiling of Human embryonic stem cell-derived neural rosettes reveal a functionally distinct early neural stem cell stage

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Project description:Purpose: The goal of this study to use ethanol-exposed human embryonic stem cell (hESC)-derived neural cells as models to investigate microRNA expression changes in the brains of subjects with alcohol use disorder (AUD). Methods: hESCs were differentiated into neural cells (mainly cortical interneurons), which were then cultured in media with or without ethanol (50-100 mM) for 7 days (by duplicate experiments). Total RNAs were extracted from hESC-derived neural cells (with or without ethanol exposure) for small RNA sequencing. The sequence reads were processed using the Comprehensive Analysis Pipeline for miRNA Sequencing Data (CAP-miRseq) workflow. Ethanol-induced miRNA transcriptomic changes were analyzed by the Limma-Voom method. Results: A 7-day ethanol exposure led to differential expression of six miRNAs (absolute FC>2.0 & P<0.05) in hESC-derived cortical interneurons. Three miRNAs were upregulated (>2-fold increase & P<0.05), while three other miRNAs were downregulated (> 2-fold decrease & P < 0.05) due to ethanol exposure. Conclusions: The hESC-derived neural cell model study can partially validate miRNA transcriptomic changes in postmortem brains of subjects with alcohol use disorder.

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