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Homo sapiens

ABSTRACT: Global transcriptional profiling changes upon knockdown of LKB1 in human glioblastoma cell lines

PROVIDER: PRJNA356645 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5085980.fastq.gz	Fastqsanger.gz
	SRR5085981.fastq.gz	Fastqsanger.gz
	SRR5085982.fastq.gz	Fastqsanger.gz
	SRR5085975.fastq.gz	Fastqsanger.gz
	SRR5085976.fastq.gz	Fastqsanger.gz

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Global transcriptional profiling changes upon knockdown of LKB1 in human glioblastoma cell lines

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Gene expression profiling associated with knockdown of LKB1 in human intrahepatic cholangiocarcinoma

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Expression data from human lung cancer cell lines with NEDD9 overexpression or NEDD9 knockdown

Project description:NEDD9 is important for lung cancer metastasis. However, the detailed mechanism remains elusive. Using the microarray data generated with human lung cancer cell lines with either NEDD9 overexpression or NEDD9 knockdown, we plan to idnetify important signal pathways regulated by NEDD9. This may explain how NEDD9 excutes its function in lung cancer. We used microarrays to detail the global programme of gene expression underlying cellularisation and identified distinct classes of up-regulated genes during this process. Human lung cancer cell line A549, which has LKB1 loss-of-function mutation and increased expression of NEDD9, was used for two individual NEDD9 knockdown. Human lung cancer cell line CRL-5907, which has wild-type LKB1 and low NEDD9 expression level, was used for NEDD9 overexpression. The microarray was done in A549 cells, A549 cells with two different NEDD9 knockdown; CRL-5907 cells and CRL-5907 cells with NEDD9 overexpression.

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Analysis of hippo pathway upon YAP knockdown and MEK inhibition

Project description:This experiment analyzes the global proteome and phosphoproteome in YAP amplified cell lines upon YAP knockdown and/or MEK inhibition.

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Gene expression changes induced by LKB1 expression in human lung adenocarcinoma cell lines

Project description:LKB1 is a tumor suppressor lost in approximately 30% of lung adenocarcinomas. It is a serine-threonine kinase involved in regulating metabolism, proliferation, and cell polarity. We have characterized its association with mRNA expression profiles in resected tumors and in cell lines, but little is known about the direct effects of LKB1 on the regulation of these genes. This study investigates the effects of LKB1 activity on mRNA expression in two LKB1-mutant lung adenocarcinoma cell lines, H2122 and A549. Wild-type LKB1 has been stably expressed in these cell lines using a pBABE retrovirus as well as an empty pBABE control and a kinase-dead mutant of LKB1 (K78I) control (Addgene). Samples submitted are two cell lines, three experimental conditions, and three replicates, for a total of 17 samples (one sample was excluded for poor RNA quality). Gene expression of these samples are analyzed to determine transcriptional regulatory effects of LKB1 expression. Results of this analysis are compared to our analysis of resected human tumors to determine gene patterns that are differentially expressed between LKB1-deficient and LKB1-wild-type tumors whose expression is also affected by restoration of LKB1 in vitro. RMA gene expression was taken from two cell lines stably expressing LKB1 or controls of K78I mutant LKB1 or empty pBABE vector. Log2 average expression differences are calculated and compared to results from analysis of gene expression associated with LKB1 loss in resected human tumors.

2013-10-01 | E-GEOD-51266 | biostudies-arrayexpress

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Project description:Transcriptional profiling of human cancer cell lines upon ZMPSTE24 silencing

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