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Promoter-proximal pausing mediated by the exon junction complex regulates splicing

ABSTRACT: Promoter-proximal pausing mediated by the exon junction complex regulates splicing

PROVIDER: PRJNA357409 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR5110080.fastq.gz	Fastqsanger.gz
	SRR5110090.fastq.gz	Fastqsanger.gz
	SRR5110250.fastq.gz	Fastqsanger.gz
	SRR5110310_1.fastq.gz	Fastqsanger.gz
	SRR5110310_2.fastq.gz	Fastqsanger.gz

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Promoter-proximal pausing mediated by the exon junction complex regulates splicing

Project description:Promoter-proximal pausing of RNA polymerase II (Pol II) is a widespread transcriptional regulatory step across metazoans. Here we find that the nuclear exon junction complex (pre-EJC) is a critical and conserved regulator of this process. Depletion of pre-EJC subunits leads to a global decrease in Pol II pausing and to premature entry into elongation. This effect occurs, at least in part, via non-canonical recruitment of pre-EJC components at promoters. Failure to recruit the pre-EJC at promoters results in increased binding of the positive transcription elongation complex (P-TEFb) and in enhanced Pol II release. Notably, restoring pausing is sufficient to rescue exon skipping and the photoreceptor differentiation defect associated with depletion of pre-EJC components in vivo. We propose that the pre-EJC serves as an early transcriptional checkpoint to prevent premature entry into elongation, ensuring proper recruitment of RNA processing components that are necessary for exon definition.

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