Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Homo sapiens

ABSTRACT: High depth sequencing of BrU-labeled nascent RNA.

PROVIDER: PRJNA358055 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR5120194_1.fastq.gz	Fastqsanger.gz
	SRR5120194_2.fastq.gz	Fastqsanger.gz
	SRR5120195_1.fastq.gz	Fastqsanger.gz
	SRR5120195_2.fastq.gz	Fastqsanger.gz
	SRR5120196_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 8

Similar Datasets

High depth sequencing of BrU-labeled nascent RNA in METTL3 depleted cells.

Project description:High depth sequencing of BrU-labeled nascent RNA in METTL3 depleted cells.

| PRJNA471827 | ENA

BrU-labeled nascent RNA sequencing and m6A-IP from BrU-labeled nascent RNA

Project description:BrU-labeled nascent RNA sequencing and m6A-IP from BrU-labeled nascent RNA

| PRJNA326350 | ENA

M6A IP from BrU-labeled and fragmented nascent RNA.

Project description:M6A immunoprecipitation from 15min BrU labeled and fragmented nascent RNA.

2018-06-19 | GSE114543 | GEO

M6A IP from BrU-labeled nascent RNA (non-fragmented).

Project description:M6A immunoprecipitation from 15 min BrU-labeled nascent RNA (0 min and 30 min chase). All three fractions, Input, eluate and supernatant were subjected to Illumina sequencing.

2018-06-19 | GSE114554 | GEO

M6A IP from BrU-labeled and fragmented nascent RNA.

Project description:M6A IP from BrU-labeled and fragmented nascent RNA.

| PRJNA471963 | ENA

M6A IP from BrU-labeled nascent RNA (non-fragmented).

Project description:M6A IP from BrU-labeled nascent RNA (non-fragmented).

| PRJNA471962 | ENA

High depth sequencing of BrU-labeled nascent RNA.

Project description:BrU incorporation into nascent RNA followed by immunoprecipitation with a BrU specific antibody. The nascent RNA was subsequently eluted with BrU competition.

2017-08-22 | GSE92565 | GEO

High depth sequencing of BrU-labeled nascent RNA in METTL3 depleted cells.

Project description:BrU incorporation into nascent RNA followed by immunoprecipitation with a BrU specific antibody. The nascent RNA was subsequently eluted with BrU competition.

2018-06-19 | GSE114542 | GEO

Homo sapiens whole blood.

Project description:RNA extracted at 240min. Samples are rRNA depleted. Expression measurement of Homo sapiens genes.

2019-07-31 | GSE114179 | GEO

Crystal structure of Homo sapiens kynureninase.

Project description:Kynureninase is a member of a large family of catalytically diverse but structurally homologous pyridoxal 5'-phosphate (PLP) dependent enzymes known as the aspartate aminotransferase superfamily or alpha-family. The Homo sapiens and other eukaryotic constitutive kynureninases preferentially catalyze the hydrolytic cleavage of 3-hydroxy-l-kynurenine to produce 3-hydroxyanthranilate and l-alanine, while l-kynurenine is the substrate of many prokaryotic inducible kynureninases. The human enzyme was cloned with an N-terminal hexahistidine tag, expressed, and purified from a bacterial expression system using Ni metal ion affinity chromatography. Kinetic characterization of the recombinant enzyme reveals classic Michaelis-Menten behavior, with a Km of 28.3 +/- 1.9 microM and a specific activity of 1.75 micromol min-1 mg-1 for 3-hydroxy-dl-kynurenine. Crystals of recombinant kynureninase that diffracted to 2.0 A were obtained, and the atomic structure of the PLP-bound holoenzyme was determined by molecular replacement using the Pseudomonas fluorescens kynureninase structure (PDB entry 1qz9) as the phasing model. A structural superposition with the P. fluorescens kynureninase revealed that these two structures resemble the "open" and "closed" conformations of aspartate aminotransferase. The comparison illustrates the dynamic nature of these proteins' small domains and reveals a role for Arg-434 similar to its role in other AAT alpha-family members. Docking of 3-hydroxy-l-kynurenine into the human kynureninase active site suggests that Asn-333 and His-102 are involved in substrate binding and molecular discrimination between inducible and constitutive kynureninase substrates.

| S-EPMC2531291 | biostudies-literature

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data