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Escherichia coli str. K-12 substr. MG1655

ABSTRACT: A bacteriophage enzyme induces bacterial metabolic perturbation that confers a novel promiscuous function.

PROVIDER: PRJNA358097 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5121110_1.fastq.gz	Fastqsanger.gz
	SRR5121110_2.fastq.gz	Fastqsanger.gz
	SRR5121111_1.fastq.gz	Fastqsanger.gz
	SRR5121111_2.fastq.gz	Fastqsanger.gz
	SRR5121112_1.fastq.gz	Fastqsanger.gz

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A bacteriophage enzyme induces bacterial metabolic perturbation that confers a novel promiscuous function.

Project description:One key concept in the evolution of new functions is the ability of enzymes to perform promiscuous side-reactions that serve as a source of novelty that may become beneficial under certain conditions. Here, we identify a mechanism where a bacteriophage-encoded enzyme introduces novelty by inducing expression of a promiscuous bacterial enzyme. By screening for bacteriophage DNA that rescued an auxotrophic E. coli mutant carrying a deletion of the ilvA gene, we show that bacteriophage-encoded S-adenosylmethionine (SAM) hydrolases reduce SAM levels. Via this perturbation of bacterial metabolism, expression of the promiscuous bacterial enzyme MetB is increased, which in turn complements the absence of IlvA. These results demonstrate how foreign DNA can increase the metabolic capacity of bacteria, not only by transfer of bona fide new genes, but also by bringing cryptic bacterial functions to light via perturbations of cellular physiology.

2018-04-11 | GSE92601 | GEO

A bacteriophage enzyme induces bacterial metabolic perturbation that confers a novel promiscuous function

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Escherichia coli str. K-12 substr. MG1655

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RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655

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RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655

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Escherichia coli str. K-12 substr. MG1655

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YAMAT-seq of mature tRNA pools in the bacterium Escherichia coli str. K-12 substr. MG1655 and derivatives

Project description:Mature tRNA pools were measured using an adaptation of YAMAT-seq (Shigematsu et al., 2017; doi:10.1093/nar/gkx005 ) and further described in (Ayan et al., 2020; doi:10.7554/eLife.57947) in 10 strain-medium combinations (all strains dervied from the model bacterium E. coli MG1655). The aim of the experiment was to investigate the effect of reducing tRNA gene copy number on mature tRNA pools in rich and poor media.

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