Project description:Recently, long noncoding rnas (lncRNAs) have been shown to have key roles in the development and prgression of hepatocellular carcinoma (HCC). However, the mechanism that contributes to the HCC biology is unknown, especially at the early and middle stages. In this study, we comprehensively investigaged lnRNAs and mRNAs expression in HCC. We found three significant lnRNAs for diagostic markers in HCC at early stage.
Project description:We conducted a quantitative assessment of drug-inducible cis-regulatory elements (CREs) based on transcription initiation profiling of mRNAs and noncoding RNAs, including enhancer RNAs, by using CAGE (Cap Analysis of Gene Expression). Candidate CREs were identified in a hepatocellular carcinoma HepG2 cell line with stable expression of drug-responsive transcription factor pregnane X receptor (PXR) .
Project description:To identifythe the functional roles and the pathophysiological contributions of coding genes and noncoding RNAs in human hepatic carcinogenesis, we analysed the differenial expression of genes and noncoding RNAs in hepatocellular carcinoma tissues and the corresponding non-cancerous tissues. We used microarrays to detail the global programme of gene expression in human hepatocellular carcinomas and correponding non-cancerous tissues.
Project description:Accumulating evidence indicates that long noncoding RNAs can interact with microRNAs to regulate target mRNAs through competing interactions. However, this mechanism remains largely unexplored in laryngeal squamous cell carcinoma. In this study, transcriptome-wide RNA sequencing in 3 pairs of laryngeal squamous cell carcinoma tissues and adjacent normal tissues was performed to investigate the expression profiles of lncRNAs, miRNAs and mRNAs.
Project description:To explore the target genes of long noncoding RNA lncTCF7, we established lncTCF7-silenced HCC primary CSC cells and conducted transcriptome microarray analysis. We used microarrays to identify distinct gene expression underlying shCtrl and shlncTCF7 of hepatocellular carcinoma sample stem cells. We cultured shlncTCF7 and shCtrl cells from hepatocellular carcinoma (HCC) clinical sample, then hybridized on Affymetrix microarrays. We sought to identify distinct target genes of lncTCF7 in liver cancer stem cells (CSCs).
Project description:To determine the role of HNF1α on long noncoding RNAs (lncRNAs) and the overall mechanisms in hepatocellular carcinoma, we analyzed the expression profiles of lncRNAs and mRNAs in huh7 cells with overexpression or knockdown of HNF1α. Expression profile showed that there were 339 mRNAs and 22 intergenic lncRNAs positively regulated by HNF1α for more than 2 times. The up-regulated genes separated samples by comparing lenti-HNF1α group with lenti-ctrl group, with overlap the down-regulated genes separated in HNF1α knockdown groups were identified as the genes positived regulated by HNF1α. Expression of seven genes from these 22 intergenic lncRNAs was quantified in the same RNA samples by real-time PCR, confirming the regulatory effect of HNF1α on lncRNAs as well as the microarray analysis pattern.
