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Transcriptomics of siCTRL, siNSUN2 and ALYREF-RIP HeLa cells, and multiple mouse tissues

ABSTRACT: Transcriptomics of siCTRL, siNSUN2 and ALYREF-RIP HeLa cells, and multiple mouse tissues

OTHER RELATED OMICS DATASETS IN: GSE93750GSE93749GSE93751

PROVIDER: PRJNA362513 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5183490_1.fastq.gz	Fastqsanger.gz
	SRR5183490_2.fastq.gz	Fastqsanger.gz
	SRR5183500_1.fastq.gz	Fastqsanger.gz
	SRR5183500_2.fastq.gz	Fastqsanger.gz
	SRR5183510_1.fastq.gz	Fastqsanger.gz

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Transcriptomics of siCTRL, siNSUN2 and ALYREF-RIP HeLa cells, and multiple mouse tissues

Project description:RNA was isolated from siCTRL, siNSUN2 and ALYREF-RIP HeLa cells, and multiple mouse tissues using the TRIzol (Invitrogen) reagent by following the company manual. Approximately 2.5 µg of total RNA was then used for library preparation using a TruSeq™ RNA Sample Prep Kit v2 (Illumina, San Diego, CA, USA) according to the manufacturer’s protocol.The libraries were sequenced using HiSeq3000 (Illumina) or HiSeq2500 in paired-read mode, creating reads with a length of 101 or 125 bp. Sequencing chemistry v2 or v4 (Illumina) was used.

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