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Homo sapiens

ABSTRACT: A next generation sequencing based approach to identify extracellular vesicle mediated mRNA transfers between cells

PROVIDER: PRJNA369094 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5209930_1.fastq.gz	Fastqsanger.gz
	SRR5209930_2.fastq.gz	Fastqsanger.gz
	SRR5209931_1.fastq.gz	Fastqsanger.gz
	SRR5209931_2.fastq.gz	Fastqsanger.gz
	SRR5209932_1.fastq.gz	Fastqsanger.gz

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Project description:Purpose: Exosomes and other extracellular vesicles (EVs) have emerged as an important mechanism of cell-to-cell communication. In this work, we present a novel next generation of sequencing (NGS) based approach to identify EV mediated mRNA exchanges between co-cultured adipocyte and macrophage cells. Methods:We performed molecular and genomic profiling and jointly considered data from RNA sequencing (RNA-seq) and genotyping to track the ‘sequence varying mRNAs’ transferred between cells. Results: We identified 8 mRNAs being transferred from macrophages to adipocytes and 21 mRNAs being transferred in the opposite direction. These mRNAs represented biological functions including extracellular matrix, cell adhesion, glycoprotein, and signal peptides. Conclusions: Our study sheds new light on EV mediated RNA communications between adipocyte and macrophage cells, which may play a significant role in developing insulin resistance in diabetic patients. This work establishes a new method that is widely applicable to examining genetic material exchanges in many other cellular systems.

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Project description:Objectives: MicroRNA (miRNA) can be released to the extracellular medium and participates in neuronal communication. We investigate the mechanisms of miRNA exocytosis by vesicle fusion as a neuromodulator in a manner that are disparate from silencing gene expression. Methods: Small RNA sequencing data of large dense-core vesicle were generated by next-generation sequencing (NGS) in triplicate using Illumina Hiseq 2500. Results: Large dense-core vesicles contain a variety of known and novel miRNAs inside including miR-375. Conclusion: miRNAs can be novel neuromodulators, which are stored in LDCVs and released by vesicle fusion by SNARE assembly and synaptotagmin-1

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2019-03-07 | GSE126419 | GEO

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