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Taibaiella chishuiensis strain:CGMCC 1.12700

ABSTRACT: Taibaiella chishuiensis CGMCC 1.12700 genome sequencing

PROVIDER: PRJNA370101 | ENA |

REPOSITORIES: ENA

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Dataset's files

Source:

			Action	DRS
	PYGD01.dat.gz	Other
	PYGD01.fasta.gz	Fasta.gz
	PYGD01.master.dat	Other
	SRR5852206_1.fastq.gz	Fastqsanger.gz
	SRR5852206_2.fastq.gz	Fastqsanger.gz

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Glutamine synthetase gene glnA plays an important role in curdlan biosynthesis of Agrobacterium sp. CGMCC 11546

Project description:Purpose： The goal of this study is compare the effect of glnA gene in curdlan synthesis in Agrobacterium sp. CGMCC 11546. methods: The transcriptional and metabolomics analysis the function of glnA in Agrobacterium sp. CGMCC 11546. Results: The transcriptional and metabolomics showed that the decrease of curdlan production in the ΔglnA mutants may be caused by the insufficient supply of energy ATP conclusion: glnA play an important role in curdlan synthesis in Agrobacterium sp. CGMCC 11546

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Methionine biosynthetic pathway genes metH and metZ affect curdlan synthesis in Agrobacterium sp. CGMCC 11546

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A Novel Nutrient Deprivation-Induced Neonicotinoid Insecticide Acetamiprid Degradation by Ensifer adhaerens CGMCC 6315

Project description:Proteomic Analysis. The proteomic expression of CGMCC 6315 under different nutrient concentration conditions was investigated by isobaric tags for relative and absolute quantitation (iTRAQ)-based quantitative proteomics. The CGMCC 6315 was cultured in LB broth and 1/15LB broth as described above, after which the strains were collected by centrifugation at 10,000× g for 10 min at 4°C. Protein extraction, digestion, iTRAQ labeling and peptide fractionation were performed using the protocol described by Jin et al. 29. Protein identification was conducted using a LC-20AD nano-HPLC instrument (Shimadzu, Kyoto, Japan) equipped with a Q EXACTIVE tandem mass spectrometer (Thermo Fisher Scientific, San Jose, CA, USA) for data-dependent acquisition detection by nano-electrospray ionization. The raw MS/MS data were converted into MGF format by the thermo scientific tool Proteome Discoverer, and the exported MGF files were searched using Mascot (version 2.3.02) against the selected database containing 7546 CGMCC 6315 coding genes. The IQuant software was used for quantitative analysis of the labeled peptides with isobaric tags. Fold changes of >1.7 with p-values <0.05 were used as a cut off for differentially regulated proteins.

2018-12-12 | PXD012014 | Pride

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