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Haloarcula hispanica

ABSTRACT: Haloarcula hispanica overview

PROVIDER: PRJNA37227 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
		Other
	SRR23782251_1.fastq.gz	Fastqsanger.gz
	SRR23782251_2.fastq.gz	Fastqsanger.gz
	SRR23782252_1.fastq.gz	Fastqsanger.gz
	SRR23782252_2.fastq.gz	Fastqsanger.gz

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Genome-wide binding of TrmB in Haloarcula hispanica

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Project description:Transcriptional profiling of Haloarcula hispanica wild-type strain cultured in different media comparing MG medium with 168 medium. H. hispanica wild-type strain can accumulate PHBV in MG medium, while can not in 168 medium. Goal was to explore the PHA biosynthesis and its impact on central metabolism in H. hispanica. Total RNA from MG and 168 were used to generate target cDNA, and then hybridized to 8*15K Haloarcula hispanica genome array genechips, representing about 3800 genes.

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Genes differentially expressed Haloarcula hispanica strains: PHB (M-NM-^TphaEC mutant) vs 960 (wild type)

Project description:Transcriptional profiling of Haloarcula hispanica comparing PHB with 960. PHB represents the PHA synthase genes mutant (i.e., M-NM-^TphaEC) which is deficient in PHBV accumulation when grown in MG medium. 960 represents the wild-type strain which can produce PHBV when grown in MG medium. Goal was to explore the PHBV biosynthesis and its impact on central metabolism in H. hispanica. Total RNA from PHB and 960 were used to generate target cDNA, and then hybridized to 8*15K Haloarcula hispanica genome array genechips, representing about 3800 genes.

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