Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

A chemical screen in zebrafish embryonic cells establishes that Akt activation is required for neural crest development

ABSTRACT: A chemical screen in zebrafish embryonic cells establishes that Akt activation is required for neural crest development

PROVIDER: PRJNA378502 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR5320540_1.fastq.gz	Fastqsanger.gz
	SRR5320540_2.fastq.gz	Fastqsanger.gz
	SRR5320541_1.fastq.gz	Fastqsanger.gz
	SRR5320541_2.fastq.gz	Fastqsanger.gz
	SRR5320542_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 24

Similar Datasets

A chemical screen in zebrafish embryonic cells establishes that Akt activation is required for neural crest development [RNA-seq]

Project description:The neural crest is a dynamic progenitor cell population that arises at the border of neural and non-neural ectoderm. The inductive roles of FGF, Wnt, and BMP at the neural plate border are well established, but the signals required for subsequent neural crest development remain poorly characterized. Here, we conducted a screen in primary zebrafish embryo cultures for chemicals that decrease neural crest formation, as read out by crestin:EGFP expression. We found that the natural product caffeic acid phenethyl ester (CAPE) disrupts neural crest gene expression, migration, and melanocytic differentiation by reducing Sox10 activity. CAPE inhibits PI3K/Akt signaling specifically in FGF-stimulated cells, and neural crest defects in CAPE-treated embryos are suppressed by constitutively active Akt1. Inhibition of Akt activity by constitutively active PTEN similarly decreases crestin expression and Sox10 activity. Our study has identified Akt as a novel intracellular pathway required for neural crest development.

2017-08-17 | GSE95814 | GEO

A chemical screen in zebrafish embryonic cells establishes that Akt activation is required for neural crest development [ATAC-seq]

2017-08-17 | GSE95813 | GEO

A chemical screen in zebrafish embryonic cells establishes that Akt activation is required for neural crest development.

Project description:The neural crest is a dynamic progenitor cell population that arises at the border of neural and non-neural ectoderm. The inductive roles of FGF, Wnt, and BMP at the neural plate border are well established, but the signals required for subsequent neural crest development remain poorly characterized. Here, we conducted a screen in primary zebrafish embryo cultures for chemicals that disrupt neural crest development, as read out by crestin:EGFP expression. We found that the natural product caffeic acid phenethyl ester (CAPE) disrupts neural crest gene expression, migration, and melanocytic differentiation by reducing Sox10 activity. CAPE inhibits FGF-stimulated PI3K/Akt signaling, and neural crest defects in CAPE-treated embryos are suppressed by constitutively active Akt1. Inhibition of Akt activity by constitutively active PTEN similarly decreases crestin expression and Sox10 activity. Our study has identified Akt as a novel intracellular pathway required for neural crest differentiation.

| S-EPMC5599238 | biostudies-literature

A chemical screen in zebrafish embryonic cells establishes that Akt activation is required for neural crest development

Project description:This SuperSeries is composed of the SubSeries listed below.

2017-08-17 | GSE95815 | GEO

Danio rerio

Project description:A chemical screen in zebrafish embryonic cells establishes that Akt activation is required for neural crest development [RNA-seq]

| PRJNA378505 | ENA

Danio rerio

Project description:A chemical screen in zebrafish embryonic cells establishes that Akt activation is required for neural crest development [ATAC-seq]

| PRJNA378506 | ENA

The Ednrb–Aim2–AKT axis regulates neural crest-derived melanoblast proliferation during early development

Project description:The Ednrb–Aim2–AKT axis regulates neural crest-derived melanoblast proliferation during early development

| PRJNA1153952 | ENA

An integrative CRISPR screen reveals the AP-1 transcription factor complex mediates activation of the human neural crest cell gene regulatory program

Project description:The complex interplay between signaling systems, transcription factors, and cis-regulatory elements required for development are encoded within gene regulatory networks (GRN). Although GRNs have greatly expanded our understanding of cell fate decisions during embryogenesis, they are typically assembled via a candidate-based approach, which fails to consider the tissue specific functions more broadly expressed TFs may employ. To address this, we combined a TF-focused CRISPR screen with epigenomic and transcriptional profiling of human neural crest cells. This approach unbiasedly identified several key regulators of human neural crest cell development, including the ubiquitous AP-1 transcription factor complex. Functional characterization of AP-1 demonstrated it is a major regulator of neural crest identity, working with the cell type specific TF, TFAP2A, to shape the neural crest epigenome. Interestingly, overexpression of AP-1 and TFAP2A in human embryonic stem cells is sufficient to drive neural crest identity, suggesting a critical role for AP-1 in the transition from pluripotency to more specialized stem cell states. Furthermore, we demonstrate that AP-1 serves as a nuclear effector of FGF/MAPK signaling in human neural crest cells, a previously missing link between FGF signaling and neural crest development. Our results demonstrate signaling effectors carry out cell-type specific enhancer selection and may contribute to our efforts in engineering neural crest cells for the purpose of tissue repair and regeneration.

2023-08-25 | GSE212793 | GEO

A chemical screen identifies compounds limiting the toxicity of C9ORF72 dipeptide repeats

Project description:A chemical screen identifies compounds limiting the toxicity of C9ORF72 dipeptide repeats

| PRJNA434079 | ENA

Chemical genomic screen identifies SP2509, WT hTERT specific repressor in colorectal cancer

Project description:Chemical genomic screen identifies SP2509, WT hTERT specific repressor in colorectal cancer

| PRJNA838606 | ENA