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Drosophila melanogaster

ABSTRACT: Genome-wide profiling of gene expression and transcription factors binding reveals new insights into the mechanisms of gene regulation during Drosophila spermatogenesis [DamID]

PROVIDER: PRJNA380896 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5388490_1.fastq.gz	Fastqsanger.gz
	SRR5388490_2.fastq.gz	Fastqsanger.gz
	SRR5388500_1.fastq.gz	Fastqsanger.gz
	SRR5388500_2.fastq.gz	Fastqsanger.gz
	SRR5388510_1.fastq.gz	Fastqsanger.gz

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Genome-wide profiling of gene expression and transcription factors binding reveals new insights into the mechanisms of gene regulation during Drosophila spermatogenesis [DamID]

Project description:To investigate the mechanisms of gene regulation during Drosophila spermatogenesis, we obtained genome-wide binding profiles of the proteins involved in gene regulation during drosophila spermatogenesis using germline-specific DamID-Seq approach.

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Genome-wide profiling of gene expression and transcription factors binding reveals new insights into the mechanisms of gene regulation during Drosophila spermatogenesis

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FlyORF-TaDa allows rapid generation of new lines for in vivo cell-type specific profiling of protein-DNA interactions in Drosophila melanogaster

Project description:Targeted DamID (TaDa) is an increasingly popular method of generating cell-type specific DNA binding profiles in vivo. Although sensitive and versatile, TaDa requires the generation of new transgenic fly lines for every protein that is profiled, which is both time-consuming and costly. Here, we describe the FlyORF-TaDa system for converting an existing FlyORF library of inducible open reading frames (ORFs) to TaDa lines via a genetic cross, with recombinant progeny easily identifiable by eye colour. Profiling the binding of the H3K36me3-associated chromatin protein MRG15 in larval neural stem cells using both FlyORF-TaDa and conventional TaDa demonstrates that new lines generated using this system provide accurate and highly-reproducible DamID binding profiles. Our data further show that MRG15 binds to a subset of active chromatin domains in vivo. Courtesy of the large coverage of the FlyORF library, the FlyORF-TaDa system enables the easy creation of TaDa lines for 74% of all transcription factors and chromatin modifying proteins within the Drosophila genome.

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Project description:Profiling of Lola-N genomic binding in Drosophila with DamID

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Project description:Genome-wide identification of the binding sites of the Drosophila transcription factors Achaete, Asense, E(spl)m3-HLH and Senseless in wing imaginal cells using DamID profiling. Each Dam-fusion-derived sample is compared to a control Dam-only sample. Two biological replicates were performed for sca-Asense, neur-Asense, sca-Achaete, neur-Achaete, neur-Sens and sca-E(spl)m3-HLH.

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Project description:Mapping of Polycomb binding in Drosophila larval brain tissue using DamID

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