Chroococcidiopsis thermalis
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA38135 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| Other | ||||
| SRR3926540.fastq.gz | Fastqsanger.gz | |||
| SRR3926541.fastq.gz | Fastqsanger.gz | |||
| SRR3926539.fastq.gz | Fastqsanger.gz |
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Project description:The cultivation of cyanobacteria by exploiting available in situ resources represents a possible way to supply food and oxygen to astronauts during long-term crewed missions on Mars. Here, we evaluated the possibility of cultivating the extremophile cyanobacterium Chroococcidiopsis thermalis CCALA 050 under operating conditions that should occur within a dome hosting a recently patented process to produce nutrients and oxygen on Mars. The medium adopted to cultivate this cyanobacterium, named Martian medium, was obtained using a mixture of regolith leachate and astronauts' urine simulants that would be available in situ resources whose exploitation could reduce the mission payload. The results demonstrated that C. thermalis can grow in such a medium. For producing high biomass, the best medium consisted of specific percentages (40%vol) of Martian medium and a standard medium (60%vol). Biomass produced in such a medium exhibits excellent antioxidant properties and contains significant amounts of pigments. Lipidomic analysis demonstrated that biomass contains strategic lipid classes able to help the astronauts facing the oxidative stress and inflammatory phenomena taking place on Mars. These characteristics suggest that this strain could serve as a valuable nutritional resource for astronauts.
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Project description:Chroococcidiopsis thermalis is an extremophilic cyanobacterium that is adaptable to a widerange of environments including deserts, where it can maintain viability after desiccation. This species is also capable of photoautotrophic growth under far-red light (FRL, 750 nm) The proteomic comparison in this study, in conjunction with topographical imaging of thylakoid membranes by atomic force microscopy, supports earlier findings that FRL growth is accompanied by the synthesis of alternative photosynthesis-related proteins. Under white light (WL), a number of phycobilisome (PBS) and photosystem (PS) subunits were predominant at levels 2 - 14-fold greater compared to FRL growth. Two antenna family proteins were markedly more abundant under WL, with a fold-difference of approximately 80. While WL-associated proteins persisted at relatively low abundance under FRL, an exclusive set of PBS and PS subunit isoforms was expressed under FRL. These proteins were undetectable under WL, in many cases encoded in a discrete FRL-associated gene cluster. In line with the expected higher energy capture rate under WL, complexes associated with electron transport (eg. cytochrome b6f) and the ATP synthase were 1.2 – 3.4 times more abundant than under FRL.
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