Dataset Information

NanoLC-MS/MS DDA Chroococcidiopsis thermalis PCC 7203 thylkoids WL vs. FRL

ABSTRACT: Chroococcidiopsis thermalis is an extremophilic cyanobacterium that is adaptable to a widerange of environments including deserts, where it can maintain viability after desiccation. This species is also capable of photoautotrophic growth under far-red light (FRL, 750 nm) The proteomic comparison in this study, in conjunction with topographical imaging of thylakoid membranes by atomic force microscopy, supports earlier findings that FRL growth is accompanied by the synthesis of alternative photosynthesis-related proteins. Under white light (WL), a number of phycobilisome (PBS) and photosystem (PS) subunits were predominant at levels 2 - 14-fold greater compared to FRL growth. Two antenna family proteins were markedly more abundant under WL, with a fold-difference of approximately 80. While WL-associated proteins persisted at relatively low abundance under FRL, an exclusive set of PBS and PS subunit isoforms was expressed under FRL. These proteins were undetectable under WL, in many cases encoded in a discrete FRL-associated gene cluster. In line with the expected higher energy capture rate under WL, complexes associated with electron transport (eg. cytochrome b6f) and the ATP synthase were 1.2 – 3.4 times more abundant than under FRL.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Chroococcidiopsis Thermalis Pcc 7203

SUBMITTER: Philip Jackson

LAB HEAD: ChristopherNeil Hunter

PROVIDER: PXD018129 | Pride | 2022-03-01

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20180206_PJ_Chro_FRL1.raw	Raw
	20180206_PJ_Chro_FRL2.raw	Raw
	20180206_PJ_Chro_FRL3.raw	Raw
	20180206_PJ_Chro_WL1.raw	Raw
	20180206_PJ_Chro_WL2.raw	Raw

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Publications

Changes in supramolecular organization of cyanobacterial thylakoid membrane complexes in response to far-red light photoacclimation.

MacGregor-Chatwin Craig C Nürnberg Dennis J DJ Jackson Philip J PJ Vasilev Cvetelin C Hitchcock Andrew A Ho Ming-Yang MY Shen Gaozhong G Gisriel Christopher J CJ Wood William H J WHJ Mahbub Moontaha M Selinger Vera M VM Johnson Matthew P MP Dickman Mark J MJ Rutherford Alfred William AW Bryant Donald A DA Hunter C Neil CN

Science advances 20220209 6

Cyanobacteria are ubiquitous in nature and have developed numerous strategies that allow them to live in a diverse range of environments. Certain cyanobacteria synthesize chlorophylls d and f to acclimate to niches enriched in far-red light (FRL) and incorporate paralogous photosynthetic proteins into their photosynthetic apparatus in a process called FRL-induced photoacclimation (FaRLiP). We characterized the macromolecular changes involved in FRL-driven photosynthesis and used atomic force mic ...[more]

PMID: 35138895

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